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Structure and mechanism of human diacylglycerol O-acyltransferase 1 期刊论文
NATURE, 2020, 581 (7808) : 329-+
作者:  Wu, Fan;  Zhao, Su;  Yu, Bin;  Chen, Yan-Mei;  Wang, Wen;  Song, Zhi-Gang;  Hu, Yi;  Tao, Zhao-Wu;  Tian, Jun-Hua;  Pei, Yuan-Yuan;  Yuan, Ming-Li;  Zhang, Yu-Ling;  Dai, Fa-Hui;  Liu, Yi;  Wang, Qi-Min;  Zheng, Jiao-Jiao;  Xu, Lin;  Holmes, Edward C.;  Zhang, Yong-Zhen
收藏  |  浏览/下载:24/0  |  提交时间:2020/07/03

The structure of human diacylglycerol O-acyltransferase 1, a membrane protein that synthesizes triacylglycerides, is solved with cryo-electron microscopy, providing insight into its function and mechanism of enzymatic activity.


Diacylglycerol O-acyltransferase 1 (DGAT1) synthesizes triacylglycerides and is required for dietary fat absorption and fat storage in humans(1). DGAT1 belongs to the membrane-bound O-acyltransferase (MBOAT) superfamily, members of which are found in all kingdoms of life and are involved in the acylation of lipids and proteins(2,3). How human DGAT1 and other mammalian members of the MBOAT family recognize their substrates and catalyse their reactions is unknown. The absence of three-dimensional structures also hampers rational targeting of DGAT1 for therapeutic purposes. Here we present the cryo-electron microscopy structure of human DGAT1 in complex with an oleoyl-CoA substrate. Each DGAT1 protomer has nine transmembrane helices, eight of which form a conserved structural fold that we name the MBOAT fold. The MBOAT fold in DGAT1 forms a hollow chamber in the membrane that encloses highly conserved catalytic residues. The chamber has separate entrances for each of the two substrates, fatty acyl-CoA and diacylglycerol. DGAT1 can exist as either a homodimer or a homotetramer and the two forms have similar enzymatic activity. The N terminus of DGAT1 interacts with the neighbouring protomer and these interactions are required for enzymatic activity.


  
The online competition between pro- and anti-vaccination views 期刊论文
NATURE, 2020, 582 (7811) : 230-+
作者:  Wu, Fan;  Zhao, Su;  Yu, Bin;  Chen, Yan-Mei;  Wang, Wen;  Song, Zhi-Gang;  Hu, Yi;  Tao, Zhao-Wu;  Tian, Jun-Hua;  Pei, Yuan-Yuan;  Yuan, Ming-Li;  Zhang, Yu-Ling;  Dai, Fa-Hui;  Liu, Yi;  Wang, Qi-Min;  Zheng, Jiao-Jiao;  Xu, Lin;  Holmes, Edward C.;  Zhang, Yong-Zhen
收藏  |  浏览/下载:10/0  |  提交时间:2020/07/03

Insights into the interactions between pro- and anti-vaccination clusters on Facebook can enable policies and approaches that attempt to interrupt the shift to anti-vaccination views and persuade undecided individuals to adopt a pro-vaccination stance.


Distrust in scientific expertise(1-14) is dangerous. Opposition to vaccination with a future vaccine against SARS-CoV-2, the causal agent of COVID-19, for example, could amplify outbreaks(2-4), as happened for measles in 2019(5,6). Homemade remedies(7,8) and falsehoods are being shared widely on the Internet, as well as dismissals of expert advice(9-11). There is a lack of understanding about how this distrust evolves at the system level(13,14). Here we provide a map of the contention surrounding vaccines that has emerged from the global pool of around three billion Facebook users. Its core reveals a multi-sided landscape of unprecedented intricacy that involves nearly 100 million individuals partitioned into highly dynamic, interconnected clusters across cities, countries, continents and languages. Although smaller in overall size, anti-vaccination clusters manage to become highly entangled with undecided clusters in the main online network, whereas pro-vaccination clusters are more peripheral. Our theoretical framework reproduces the recent explosive growth in anti-vaccination views, and predicts that these views will dominate in a decade. Insights provided by this framework can inform new policies and approaches to interrupt this shift to negative views. Our results challenge the conventional thinking about undecided individuals in issues of contention surrounding health, shed light on other issues of contention such as climate change(11), and highlight the key role of network cluster dynamics in multi-species ecologies(15).


  
Can Remotely Sensed Actual Evapotranspiration Facilitate Hydrological Prediction in Ungauged Regions Without Runoff Calibration? 期刊论文
WATER RESOURCES RESEARCH, 2020, 56 (1)
作者:  Zhang, Yongqiang;  Chiew, Francis H. S.;  Liu, Changming;  Tang, Qiuhong;  Xia, Jun;  Tian, Jing;  Kong, Dongdong;  Li, Congcong
收藏  |  浏览/下载:9/0  |  提交时间:2020/07/02
Constituents of Household Air Pollution and Risk of Lung Cancer among Never-Smoking Women in Xuanwei and Fuyuan, China 期刊论文
ENVIRONMENTAL HEALTH PERSPECTIVES, 2019, 127 (9)
作者:  Vermeulen, Roel;  Downward, George S.;  Zhang, Jinming;  Hu, Wei;  Portengen, Lutzen;  Bassig, Bryan A.;  Hammond, S. Katharine;  Wong, Jason Y. Y.;  Li, Jihua;  Reiss, Boris;  He, Jun;  Tian, Linwei;  Yang, Kaiyun;  Seow, Wei Jie;  Xu, Jun;  Anderson, Kim;  Ji, Bu-Tian;  Silverman, Debra;  Chanock, Stephen;  Huang, Yunchao;  Rothman, Nathaniel;  Lan, Qing
收藏  |  浏览/下载:12/0  |  提交时间:2019/11/27
MiR-497 similar to 195 cluster regulates angiogenesis during coupling with osteogenesis by maintaining endothelial Notch and HIF-1 alpha activity 期刊论文
NATURE COMMUNICATIONS, 2017, 8
作者:  Yang, Mi;  Li, Chang-Jun;  Sun, Xi;  Guo, Qi;  Xiao, Ye;  Su, Tian;  Tu, Man-Li;  Peng, Hui;  Lu, Qiong;  Liu, Qing;  He, Hong-Bo;  Jiang, Tie-Jian;  Lei, Min-Xiang;  Wan, Mei;  Cao, Xu;  Luo, Xiang-Hang
收藏  |  浏览/下载:20/0  |  提交时间:2019/11/27
Discovery of TaFeSb-based half-Heuslers with high thermoelectric performance 期刊论文
NATURE COMMUNICATIONS, 2019, 10
作者:  Zhu, Hangtian;  Mao, Jun;  Li, Yuwei;  Sun, Jifeng;  Wang, Yumei;  Zhu, Qing;  Li, Guannan;  Song, Qichen;  Zhou, Jiawei;  Fu, Yuhao;  He, Ran;  Tong, Tian;  Liu, Zihang;  Ren, Wuyang;  You, Li;  Wang, Zhiming;  Luo, Jun;  Sotnikov, Andrei;  Bao, Jiming;  Nielsch, Kornelius;  Chen, Gang;  Singh, David J.;  Ren, Zhifeng
收藏  |  浏览/下载:8/0  |  提交时间:2019/11/27
An electron transfer path connects subunits of a mycobacterial respiratory supercomplex 期刊论文
SCIENCE, 2018, 362 (6418) : 1020-+
作者:  Gong, Hongri;  Li, Jun;  Xu, Ao;  Tang, Yanting;  Ji, Wenxin;  Gao, Ruogu;  Wang, Shuhui;  Yu, Lu;  Tian, Changlin;  Li, Jingwen;  Yen, Hsin-Yung;  Lam, Sin Man;  Shui, Guanghou;  Yang, Xiuna;  Sun, Yuna;  Li, Xuemei;  Jia, Minze;  Yang, Cheng;  Jiang, Biao;  Lou, Zhiyong;  Robinson, Carol V.;  Wong, Luet-Lok;  Guddat, Luke W.;  Sun, Fei;  Wang, Quan;  Rao, Zihe
收藏  |  浏览/下载:11/0  |  提交时间:2019/11/27
The evolutionary history of vertebrate RNA viruses 期刊论文
NATURE, 2018, 556 (7700) : 197-+
作者:  Shi, Mang;  Lin, Xian-Dan;  Chen, Xiao;  Tian, Jun-Hua;  Chen, Liang-Jun;  Li, Kun;  Wang, Wen;  Eden, John-Sebastian;  Shen, Jin-Jin;  Liu, Li;  Holmes, Edward C.;  Zhang, Yong-Zhen
收藏  |  浏览/下载:13/0  |  提交时间:2019/11/27
Interannual cycles of Hantaan virus outbreaks at the human-animal interface in Central China are controlled by temperature and rainfall 期刊论文
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (30) : 8041-8046
作者:  Tian, Huaiyu;  Yu, Pengbo;  Cazelles, Bernard;  Xu, Lei;  Tan, Hua;  Yang, Jing;  Huang, Shanqian;  Xu, Bo;  Cai, Jun;  Ma, Chaofeng;  Wei, Jing;  Li, Shen;  Qu, Jianhui;  Laine, Marko;  Wang, Jingjun;  Tong, Shilu;  Stenseth, Nils Chr.;  Xu, Bing
收藏  |  浏览/下载:14/0  |  提交时间:2019/11/27
Hantaan virus  spillover to humans  wildlife reservoir  time-series data  climate change  
Plasma Perfluoroalkyl and Polyfluoroalkyl Substances Concentration and Menstrual Cycle Characteristics in Preconception Women 期刊论文
ENVIRONMENTAL HEALTH PERSPECTIVES, 2017, 125 (6)
作者:  Zhou, Wei;  Zhang, Lulu;  Tong, Chuanliang;  Fang, Fang;  Zhao, Shasha;  Tian, Ying;  Tao, Yexuan;  Zhang, Jun
收藏  |  浏览/下载:7/0  |  提交时间:2019/04/09