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Structure and mechanism of human diacylglycerol O-acyltransferase 1 期刊论文
NATURE, 2020, 581 (7808) : 329-+
作者:  Wu, Fan;  Zhao, Su;  Yu, Bin;  Chen, Yan-Mei;  Wang, Wen;  Song, Zhi-Gang;  Hu, Yi;  Tao, Zhao-Wu;  Tian, Jun-Hua;  Pei, Yuan-Yuan;  Yuan, Ming-Li;  Zhang, Yu-Ling;  Dai, Fa-Hui;  Liu, Yi;  Wang, Qi-Min;  Zheng, Jiao-Jiao;  Xu, Lin;  Holmes, Edward C.;  Zhang, Yong-Zhen
收藏  |  浏览/下载:24/0  |  提交时间:2020/07/03

The structure of human diacylglycerol O-acyltransferase 1, a membrane protein that synthesizes triacylglycerides, is solved with cryo-electron microscopy, providing insight into its function and mechanism of enzymatic activity.


Diacylglycerol O-acyltransferase 1 (DGAT1) synthesizes triacylglycerides and is required for dietary fat absorption and fat storage in humans(1). DGAT1 belongs to the membrane-bound O-acyltransferase (MBOAT) superfamily, members of which are found in all kingdoms of life and are involved in the acylation of lipids and proteins(2,3). How human DGAT1 and other mammalian members of the MBOAT family recognize their substrates and catalyse their reactions is unknown. The absence of three-dimensional structures also hampers rational targeting of DGAT1 for therapeutic purposes. Here we present the cryo-electron microscopy structure of human DGAT1 in complex with an oleoyl-CoA substrate. Each DGAT1 protomer has nine transmembrane helices, eight of which form a conserved structural fold that we name the MBOAT fold. The MBOAT fold in DGAT1 forms a hollow chamber in the membrane that encloses highly conserved catalytic residues. The chamber has separate entrances for each of the two substrates, fatty acyl-CoA and diacylglycerol. DGAT1 can exist as either a homodimer or a homotetramer and the two forms have similar enzymatic activity. The N terminus of DGAT1 interacts with the neighbouring protomer and these interactions are required for enzymatic activity.


  
The online competition between pro- and anti-vaccination views 期刊论文
NATURE, 2020, 582 (7811) : 230-+
作者:  Wu, Fan;  Zhao, Su;  Yu, Bin;  Chen, Yan-Mei;  Wang, Wen;  Song, Zhi-Gang;  Hu, Yi;  Tao, Zhao-Wu;  Tian, Jun-Hua;  Pei, Yuan-Yuan;  Yuan, Ming-Li;  Zhang, Yu-Ling;  Dai, Fa-Hui;  Liu, Yi;  Wang, Qi-Min;  Zheng, Jiao-Jiao;  Xu, Lin;  Holmes, Edward C.;  Zhang, Yong-Zhen
收藏  |  浏览/下载:10/0  |  提交时间:2020/07/03

Insights into the interactions between pro- and anti-vaccination clusters on Facebook can enable policies and approaches that attempt to interrupt the shift to anti-vaccination views and persuade undecided individuals to adopt a pro-vaccination stance.


Distrust in scientific expertise(1-14) is dangerous. Opposition to vaccination with a future vaccine against SARS-CoV-2, the causal agent of COVID-19, for example, could amplify outbreaks(2-4), as happened for measles in 2019(5,6). Homemade remedies(7,8) and falsehoods are being shared widely on the Internet, as well as dismissals of expert advice(9-11). There is a lack of understanding about how this distrust evolves at the system level(13,14). Here we provide a map of the contention surrounding vaccines that has emerged from the global pool of around three billion Facebook users. Its core reveals a multi-sided landscape of unprecedented intricacy that involves nearly 100 million individuals partitioned into highly dynamic, interconnected clusters across cities, countries, continents and languages. Although smaller in overall size, anti-vaccination clusters manage to become highly entangled with undecided clusters in the main online network, whereas pro-vaccination clusters are more peripheral. Our theoretical framework reproduces the recent explosive growth in anti-vaccination views, and predicts that these views will dominate in a decade. Insights provided by this framework can inform new policies and approaches to interrupt this shift to negative views. Our results challenge the conventional thinking about undecided individuals in issues of contention surrounding health, shed light on other issues of contention such as climate change(11), and highlight the key role of network cluster dynamics in multi-species ecologies(15).


  
In vitro culture of cynomolgus monkey embryos beyond early gastrulation 期刊论文
SCIENCE, 2019, 366 (6467) : 836-+
作者:  Ma, Huaixiao;  Zhai, Jinglei;  Wan, Haifeng;  Jiang, Xiangxiang;  Wang, Xiaoxiao;  Wang, Lin;  Xiang, Yunlong;  He, Xiechao;  Zhao, Zhen-Ao;  Zhao, Bo;  Zheng, Ping;  Li, Lei;  Wang, Hongmei
收藏  |  浏览/下载:12/0  |  提交时间:2020/02/17
A genome-wide association study identifies six novel risk loci for primary biliary cholangitis 期刊论文
NATURE COMMUNICATIONS, 2017, 8
作者:  Qiu, Fang;  Tang, Ruqi;  Zuo, Xianbo;  Shi, Xingjuan;  Wei, Yiran;  Zheng, Xiaodong;  Dai, Yaping;  Gong, Yuhua;  Wang, Lan;  Xu, Ping;  Zhu, Xiang;  Wu, Jian;  Han, Chongxu;  Gao, Yueqiu;  Zhang, Kui;  Jiang, Yuzhang;  Zhou, Jianbo;  Shao, Youlin;  Hu, Zhigang;  Tian, Ye;  Zhang, Haiyan;  Dai, Na;  Liu, Lei;  Wu, Xudong;  Zhao, Weifeng;  Zhang, Xiaomin;  Zang, Zhidong;  Nie, Jinshan;  Sun, Weihao;  Zhao, Yi;  Mao, Yuan;  Jiang, Po;  Ji, Hualiang;  Dong, Qing;  Li, Junming;  Li, Zhenzhong;  Bai, Xinli;  Li, Li;  Lin, Maosong;  Dong, Ming;  Li, Jinxin;  Zhu, Ping;  Wang, Chan;  Zhang, Yanqiu;  Jiang, Peng;  Wang, Yujue;  Jawed, Rohil;  Xu, Jing;  Zhang, Yu;  Wang, Qixia;  Yang, Yue;  Yang, Fan;  Lian, Min;  Jiang, Xiang;  Xiao, Xiao;  Li, Yanmei;  Fang, Jingyuan;  Qiu, Dekai;  Zhu, Zhen;  Qiu, Hong;  Zhang, Jianqiong;  Tian, Wenyan;  Chen, Sufang;  Jiang, Ling;  Ji, Bing;  Li, Ping;  Chen, Guochang;  Wu, Tianxue;  Sun, Yan;  Yu, Jianjiang;  Tang, Huijun;  He, Michun;  Xia, Min;  Pei, Hao;  Huang, Lihua;  Qing, Zhuye;  Wu, Jianfang;  Huang, Qinghai;  Han, Junhai;  Xie, Wei;  Sun, Zhongsheng;  Guo, Jian;  He, Gengsheng;  Gershwin, M. Eric;  Lian, Zhexiong;  Liu, Xiang;  Seldin, Michael F.;  Liu, Xiangdong;  Chen, Weichang;  Ma, Xiong
收藏  |  浏览/下载:17/0  |  提交时间:2019/11/27
Size-Resolved Characterization of the Chromophores in Atmospheric Particulate Matter From a Typical Coal-Burning City in China 期刊论文
JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES, 2019
作者:  Chen, Qingcai;  Mu, Zhen;  Song, Wenhuai;  Wang, Yuqin;  Yang, Zhaohong;  Zhang, Lixin;  Zhang, Yan-Lin
收藏  |  浏览/下载:8/0  |  提交时间:2019/11/27
atmospheric chromophores  excitation-emission matrix (EEM)  size-resolved characterizations  parallel factor analysis (PARAFAC)  
A Model Investigation of Aerosol-Induced Changes in the East Asian Winter Monsoon 期刊论文
GEOPHYSICAL RESEARCH LETTERS, 2019, 46 (16) : 10186-10195
作者:  Liu, Zhen;  Ming, Yi;  Wang, Lin;  Bollasina, Massimo;  Luo, Ming;  Lau, Ngar-Cheung;  Yim, Steve Hung-Lam
收藏  |  浏览/下载:6/0  |  提交时间:2019/11/27
A spatio-temporal delineation of trans-boundary ecosystem service flows from Inner Mongolia 期刊论文
ENVIRONMENTAL RESEARCH LETTERS, 2019, 14 (6)
作者:  Xie, Gaodi;  Liu, Jingya;  Xu, Jie;  Xiao, Yu;  Zhen, Lin;  Zhang, Changshun;  Wang, Yangyang;  Qin, Keyu;  Gan, Shuang;  Jiang, Yuan
收藏  |  浏览/下载:7/0  |  提交时间:2019/11/26
supply-demand balance  value flow  Inner Mongolia  ecosystem service flows  trans-boundary  
Possible heterogeneous chemistry of hydroxymethanesulfonate (HMS) in northern China winter haze 期刊论文
ATMOSPHERIC CHEMISTRY AND PHYSICS, 2019, 19 (2) : 1357-1371
作者:  Song, Shaojie;  Gao, Meng;  Xu, Weiqi;  Sun, Yele;  Worsnop, Douglas R.;  Jayne, John T.;  Zhang, Yuzhong;  Zhu, Lei;  Li, Mei;  Zhou, Zhen;  Cheng, Chunlei;  Lv, Yibing;  Wang, Ying;  Peng, Wei;  Xu, Xiaobin;  Lin, Nan;  Wang, Yuxuan;  Wang, Shuxiao;  Munger, J. William;  Jacob, Daniel J.;  McElroy, Michael B.
收藏  |  浏览/下载:15/0  |  提交时间:2019/04/09
Assessing the effects of ecological restoration approaches in the alpine rangelands of the Qinghai-Tibetan Plateau 期刊论文
ENVIRONMENTAL RESEARCH LETTERS, 2018, 13 (9)
作者:  Zhen, Lin;  Du, Bingzhen;  Wei, Yunjie;  Xiao, Yu;  Sheng, Wenping
收藏  |  浏览/下载:6/0  |  提交时间:2019/04/09
ecological restoration approaches  NDVI  animal grazing  income  Qinghai-Tibetan Plateau  
RAP2 mediates mechanoresponses of the Hippo pathway 期刊论文
NATURE, 2018, 560 (7720) : 655-+
作者:  Meng, Zhipeng;  Qiu, Yunjiang;  Lin, Kimberly C.;  Kumar, Aditya;  Placone, Jesse K.;  Fang, Cao;  Wang, Kuei-Chun;  Lu, Shicong;  Pan, Margaret;  Hong, Audrey W.;  Moroishi, Toshiro;  Luo, Min;  Plouffe, Steven W.;  Diao, Yarui;  Ye, Zhen;  Park, Hyun Woo;  Wang, Xiaoqiong;  Yu, Fa-Xing;  Chien, Shu;  Wang, Cun-Yu;  Ren, Bing;  Engler, Adam J.;  Guan, Kun-Liang
收藏  |  浏览/下载:8/0  |  提交时间:2019/11/27