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DOI | 10.1126/science.aax7890 |
In vitro culture of cynomolgus monkey embryos beyond early gastrulation | |
Ma, Huaixiao1,2,3; Zhai, Jinglei1,3,4; Wan, Haifeng1,3; Jiang, Xiangxiang1,3; Wang, Xiaoxiao1,3,4; Wang, Lin2; Xiang, Yunlong1; He, Xiechao5; Zhao, Zhen-Ao1; Zhao, Bo2; Zheng, Ping2,6; Li, Lei1,3; Wang, Hongmei1,3,4 | |
2019-11-15 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 366期号:6467页码:836-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
英文摘要 | Gastrulation is a key event in embryonic development when the germ layers are specified and the basic animal body plan is established. The complexities of primate gastrulation remain a mystery because of the difficulties in accessing primate embryos at this stage. Here, we report the establishment of an in vitro culture (IVC) system that supports the continuous development of cynomolgus monkey blastocysts beyond early gastrulation up to 20 days after fertilization. The IVC embryos highly recapitulated the key events of in vivo early postimplantation development, including segregation of the epiblast and hypoblast, formation of the amniotic and yolk sac cavities, appearance of the primordial germ cells, and establishment of the anterior-posterior axis. Single-cell RNA-sequencing analyses of the IVC embryos provide information about lineage specification during primate early postimplantation development. This system provides a platform with which to explore the characteristics and mechanisms of early postimplantation embryogenesis in primates with possible conservation of cell movements and lineages in human embryogenesis. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000496946200045 |
WOS关键词 | BLASTOCYST DIFFERENTIATION ; LINEAGE ALLOCATION ; SELF-ORGANIZATION ; MOUSE ; CELLS ; MORPHOGENESIS ; IMPLANTATION ; EXPRESSION ; COORDINATE ; MATRIGEL |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/226229 |
专题 | 环境与发展全球科技态势 |
作者单位 | 1.Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Beijing 100101, Peoples R China; 2.Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650223, Yunnan, Peoples R China; 3.Chinese Acad Sci, Innovat Acad Stem Cell & Regenerat, Beijing 100101, Peoples R China; 4.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Zool, Beijing 100049, Peoples R China; 5.Chinese Acad Sci, Kunming Inst Zool, Primate Res Ctr, Yunnan Key Lab Anim Reprod, Kunming 650223, Yunnan, Peoples R China; 6.Chinese Acad Sci, Ctr Excellence Anim Evolut & Genet, Kunming 650223, Yunnan, Peoples R China |
推荐引用方式 GB/T 7714 | Ma, Huaixiao,Zhai, Jinglei,Wan, Haifeng,et al. In vitro culture of cynomolgus monkey embryos beyond early gastrulation[J]. SCIENCE,2019,366(6467):836-+. |
APA | Ma, Huaixiao.,Zhai, Jinglei.,Wan, Haifeng.,Jiang, Xiangxiang.,Wang, Xiaoxiao.,...&Wang, Hongmei.(2019).In vitro culture of cynomolgus monkey embryos beyond early gastrulation.SCIENCE,366(6467),836-+. |
MLA | Ma, Huaixiao,et al."In vitro culture of cynomolgus monkey embryos beyond early gastrulation".SCIENCE 366.6467(2019):836-+. |
条目包含的文件 | 条目无相关文件。 |
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