Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies

doi:10.1126/science.abd0831

GSTDTAP > 气候变化

DOI	10.1126/science.abd0831
	Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies
	Alina Baum; Benjamin O. Fulton; Elzbieta Wloga; Richard Copin; Kristen E. Pascal; Vincenzo Russo; Stephanie Giordano; Kathryn Lanza; Nicole Negron; Min Ni; Yi Wei; Gurinder S. Atwal; Andrew J. Murphy; Neil Stahl; George D. Yancopoulos; Christos A. Kyratsous
	2020-08-21
发表期刊	Science
出版年	2020
英文摘要	There is an urgent focus on antibodies that target the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral spike and prevent the virus from entering host cells. Hansen et al. generated a large panel of antibodies against the spike protein from humanized mice and recovered patients. From this panel, they identified several neutralizing antibodies, including pairs that do not compete for binding to the receptor binding domain. Baum et al. focused in on four of these antibodies. All four are effective against known spike variants. However, by growing a pseudovirus that expresses the spike in the presence of individual antibodies, the authors were able to select for spike mutants resistant to that antibody. In contrast, escape mutants are not selected when pseudovirus is grown in the presence of pairs of antibodies that either do not compete or only partially compete for binding to the RBD. Such a pair might be used in a therapeutic antibody cocktail. Science , this issue p. [1010][1], p. [1014][2] Antibodies targeting the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) present a promising approach to combat the coronavirus disease 2019 (COVID-19) pandemic; however, concerns remain that mutations can yield antibody resistance. We investigated the development of resistance against four antibodies to the spike protein that potently neutralize SARS-CoV-2, individually as well as when combined into cocktails. These antibodies remain effective against spike variants that have arisen in the human population. However, novel spike mutants rapidly appeared after in vitro passaging in the presence of individual antibodies, resulting in loss of neutralization; such escape also occurred with combinations of antibodies binding diverse but overlapping regions of the spike protein. Escape mutants were not generated after treatment with a noncompeting antibody cocktail. [1]: /lookup/doi/10.1126/science.abd0827 [2]: /lookup/doi/10.1126/science.abd0831
领域	气候变化 ; 资源环境
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引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/291226
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Alina Baum,Benjamin O. Fulton,Elzbieta Wloga,et al. Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies[J]. Science,2020.
APA	Alina Baum.,Benjamin O. Fulton.,Elzbieta Wloga.,Richard Copin.,Kristen E. Pascal.,...&Christos A. Kyratsous.(2020).Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies.Science.
MLA	Alina Baum,et al."Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies".Science (2020).