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DOI | 10.1126/science.aah3605 |
Optical control of cell signaling by single-chain photoswitchable kinases | |
Zhou, Xin X.1; Fan, Linlin Z.1,5; Li, Pengpeng2,3,6; Shen, Kang2,3; Lin, Michael Z.1,4 | |
2017-02-24 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 355期号:6327页码:836-841 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Protein kinases transduce signals to regulate a wide array of cellular functions in eukaryotes. A generalizable method for optical control of kinases would enable fine spatiotemporal interrogation or manipulation of these various functions. We report the design and application of single-chain cofactor-free kinases with photoswitchable activity. We engineered a dimeric protein, pdDronpa, that dissociates in cyan light and reassociates in violet light. Attaching two pdDronpa domains at rationally selected locations in the kinase domain, we created the photoswitchable kinases psRaf1, psMEK1, psMEK2, and psCDK5. Using these photoswitchable kinases, we established an all-optical cell-based assay for screening inhibitors, uncovered a direct and rapid inhibitory feedback loop from ERK to MEK1, and mediated developmental changes and synaptic vesicle transport in vivo using light. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000395127600041 |
WOS关键词 | PROTEIN-PROTEIN INTERACTIONS ; RECEPTOR TYROSINE KINASES ; LIVING CELLS ; SPATIOTEMPORAL CONTROL ; MAMMALIAN-CELLS ; GENE-EXPRESSION ; OPTOGENETIC CONTROL ; MEDIATED INDUCTION ; NEGATIVE-FEEDBACK ; BLUE-LIGHT |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/195502 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA; 2.Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA; 3.Stanford Univ, Dept Biol, Stanford, CA 94305 USA; 4.Stanford Univ, Dept Neurobiol, Stanford, CA 94305 USA; 5.Harvard Univ, Program Chem Biol, Cambridge, MA 02138 USA; 6.Harvard Med Sch, Boston Childrens Hosp, Boston, MA USA |
推荐引用方式 GB/T 7714 | Zhou, Xin X.,Fan, Linlin Z.,Li, Pengpeng,et al. Optical control of cell signaling by single-chain photoswitchable kinases[J]. SCIENCE,2017,355(6327):836-841. |
APA | Zhou, Xin X.,Fan, Linlin Z.,Li, Pengpeng,Shen, Kang,&Lin, Michael Z..(2017).Optical control of cell signaling by single-chain photoswitchable kinases.SCIENCE,355(6327),836-841. |
MLA | Zhou, Xin X.,et al."Optical control of cell signaling by single-chain photoswitchable kinases".SCIENCE 355.6327(2017):836-841. |
条目包含的文件 | 条目无相关文件。 |
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