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The lysosomal Rag-Ragulator complex licenses RIPK1– and caspase-8–mediated pyroptosis by Yersinia 期刊论文
Science, 2021
作者:  Zengzhang Zheng;  Wanyan Deng;  Yang Bai;  Rui Miao;  Shenglin Mei;  Zhibin Zhang;  Youdong Pan;  Yi Wang;  Rui Min;  Fan Deng;  Zeyu Wu;  Wu Li;  Pengcheng Chen;  Tianchi Ma;  Xiwen Lou;  Judy Lieberman;  Xing Liu
收藏  |  浏览/下载:11/0  |  提交时间:2021/07/27
New particle formation in the remote marine boundary layer 期刊论文
Nature Communications, 2021
作者:  Guangjie Zheng;  Yang Wang;  Robert Wood;  Michael P. Jensen;  Chongai Kuang;  Isabel L. McCoy;  Alyssa Matthews;  Fan Mei;  Jason M. Tomlinson;  John E. Shilling;  Maria A. Zawadowicz;  Ewan Crosbie;  Richard Moore;  Luke Ziemba;  Meinrat O. Andreae;  Jian Wang
收藏  |  浏览/下载:10/0  |  提交时间:2021/01/28
Elevated atmospheric CO2 reduces yield‐scaled N2O fluxes from subtropical rice systems: Six site‐years field experiments 期刊论文
Global Change Biology, 2020
作者:  Zhisheng Yao;  Rui Wang;  Xunhua Zheng;  Baoling Mei;  Zaixing Zhou;  Baohua Xie;  Haibo Dong;  Chunyan Liu;  Shenghui Han;  Zhongjun Xu;  Klaus Butterbach‐;  Bahl;  Jianguo Zhu
收藏  |  浏览/下载:14/0  |  提交时间:2020/11/20
Methylmercury produced in upper oceans accumulates in deep Mariana Trench fauna 期刊论文
NATURE COMMUNICATIONS, 2020, 11 (1)
作者:  Sun, Ruoyu;  Yuan, Jingjing;  Sonke, Jeroen E.;  Zhang, Yanxu;  Zhang, Tong;  Zheng, Wang;  Chen, Shun;  Meng, Mei;  Chen, Jiubin;  Liu, Yi;  Peng, Xiaotong;  Liu, Congqiang
收藏  |  浏览/下载:10/0  |  提交时间:2020/07/09
Structure and mechanism of human diacylglycerol O-acyltransferase 1 期刊论文
NATURE, 2020, 581 (7808) : 329-+
作者:  Wu, Fan;  Zhao, Su;  Yu, Bin;  Chen, Yan-Mei;  Wang, Wen;  Song, Zhi-Gang;  Hu, Yi;  Tao, Zhao-Wu;  Tian, Jun-Hua;  Pei, Yuan-Yuan;  Yuan, Ming-Li;  Zhang, Yu-Ling;  Dai, Fa-Hui;  Liu, Yi;  Wang, Qi-Min;  Zheng, Jiao-Jiao;  Xu, Lin;  Holmes, Edward C.;  Zhang, Yong-Zhen
收藏  |  浏览/下载:24/0  |  提交时间:2020/07/03

The structure of human diacylglycerol O-acyltransferase 1, a membrane protein that synthesizes triacylglycerides, is solved with cryo-electron microscopy, providing insight into its function and mechanism of enzymatic activity.


Diacylglycerol O-acyltransferase 1 (DGAT1) synthesizes triacylglycerides and is required for dietary fat absorption and fat storage in humans(1). DGAT1 belongs to the membrane-bound O-acyltransferase (MBOAT) superfamily, members of which are found in all kingdoms of life and are involved in the acylation of lipids and proteins(2,3). How human DGAT1 and other mammalian members of the MBOAT family recognize their substrates and catalyse their reactions is unknown. The absence of three-dimensional structures also hampers rational targeting of DGAT1 for therapeutic purposes. Here we present the cryo-electron microscopy structure of human DGAT1 in complex with an oleoyl-CoA substrate. Each DGAT1 protomer has nine transmembrane helices, eight of which form a conserved structural fold that we name the MBOAT fold. The MBOAT fold in DGAT1 forms a hollow chamber in the membrane that encloses highly conserved catalytic residues. The chamber has separate entrances for each of the two substrates, fatty acyl-CoA and diacylglycerol. DGAT1 can exist as either a homodimer or a homotetramer and the two forms have similar enzymatic activity. The N terminus of DGAT1 interacts with the neighbouring protomer and these interactions are required for enzymatic activity.


  
The online competition between pro- and anti-vaccination views 期刊论文
NATURE, 2020, 582 (7811) : 230-+
作者:  Wu, Fan;  Zhao, Su;  Yu, Bin;  Chen, Yan-Mei;  Wang, Wen;  Song, Zhi-Gang;  Hu, Yi;  Tao, Zhao-Wu;  Tian, Jun-Hua;  Pei, Yuan-Yuan;  Yuan, Ming-Li;  Zhang, Yu-Ling;  Dai, Fa-Hui;  Liu, Yi;  Wang, Qi-Min;  Zheng, Jiao-Jiao;  Xu, Lin;  Holmes, Edward C.;  Zhang, Yong-Zhen
收藏  |  浏览/下载:10/0  |  提交时间:2020/07/03

Insights into the interactions between pro- and anti-vaccination clusters on Facebook can enable policies and approaches that attempt to interrupt the shift to anti-vaccination views and persuade undecided individuals to adopt a pro-vaccination stance.


Distrust in scientific expertise(1-14) is dangerous. Opposition to vaccination with a future vaccine against SARS-CoV-2, the causal agent of COVID-19, for example, could amplify outbreaks(2-4), as happened for measles in 2019(5,6). Homemade remedies(7,8) and falsehoods are being shared widely on the Internet, as well as dismissals of expert advice(9-11). There is a lack of understanding about how this distrust evolves at the system level(13,14). Here we provide a map of the contention surrounding vaccines that has emerged from the global pool of around three billion Facebook users. Its core reveals a multi-sided landscape of unprecedented intricacy that involves nearly 100 million individuals partitioned into highly dynamic, interconnected clusters across cities, countries, continents and languages. Although smaller in overall size, anti-vaccination clusters manage to become highly entangled with undecided clusters in the main online network, whereas pro-vaccination clusters are more peripheral. Our theoretical framework reproduces the recent explosive growth in anti-vaccination views, and predicts that these views will dominate in a decade. Insights provided by this framework can inform new policies and approaches to interrupt this shift to negative views. Our results challenge the conventional thinking about undecided individuals in issues of contention surrounding health, shed light on other issues of contention such as climate change(11), and highlight the key role of network cluster dynamics in multi-species ecologies(15).


  
Recycling and metabolic flexibility dictate life in the lower oceanic crust 期刊论文
NATURE, 2020, 579 (7798) : 250-+
作者:  Zhou, Peng;  Yang, Xing-Lou;  Wang, Xian-Guang;  Hu, Ben;  Zhang, Lei;  Zhang, Wei;  Si, Hao-Rui;  Zhu, Yan;  Li, Bei;  Huang, Chao-Lin;  Chen, Hui-Dong;  Chen, Jing;  Luo, Yun;  Guo, Hua;  Jiang, Ren-Di;  Liu, Mei-Qin;  Chen, Ying;  Shen, Xu-Rui;  Wang, Xi;  Zheng, Xiao-Shuang;  Zhao, Kai;  Chen, Quan-Jiao;  Deng, Fei;  Liu, Lin-Lin;  Yan, Bing;  Zhan, Fa-Xian;  Wang, Yan-Yi;  Xiao, Geng-Fu;  Shi, Zheng-Li
收藏  |  浏览/下载:37/0  |  提交时间:2020/05/13

The lithified lower oceanic crust is one of Earth'  s last biological frontiers as it is difficult to access. It is challenging for microbiota that live in marine subsurface sediments or igneous basement to obtain sufficient carbon resources and energy to support growth(1-3) or to meet basal power requirements(4) during periods of resource scarcity. Here we show how limited and unpredictable sources of carbon and energy dictate survival strategies used by low-biomass microbial communities that live 10-750 m below the seafloor at Atlantis Bank, Indian Ocean, where Earth'  s lower crust is exposed at the seafloor. Assays of enzyme activities, lipid biomarkers, marker genes and microscopy indicate heterogeneously distributed and viable biomass with ultralow cell densities (fewer than 2,000 cells per cm(3)). Expression of genes involved in unexpected heterotrophic processes includes those with a role in the degradation of polyaromatic hydrocarbons, use of polyhydroxyalkanoates as carbon-storage molecules and recycling of amino acids to produce compounds that can participate in redox reactions and energy production. Our study provides insights into how microorganisms in the plutonic crust are able to survive within fractures or porous substrates by coupling sources of energy to organic and inorganic carbon resources that are probably delivered through the circulation of subseafloor fluids or seawater.


  
Improved protein structure prediction using potentials from deep learning 期刊论文
NATURE, 2020, 577 (7792) : 706-+
作者:  Ma, Runze;  Cao, Duanyun;  Zhu, Chongqin;  Tian, Ye;  Peng, Jinbo;  Guo, Jing;  Chen, Ji;  Li, Xin-Zheng;  Francisco, Joseph S.;  Zeng, Xiao Cheng;  Xu, Li-Mei;  Wang, En-Ge;  Jiang, Ying
收藏  |  浏览/下载:142/0  |  提交时间:2020/07/03

Protein structure prediction can be used to determine the three-dimensional shape of a protein from its amino acid sequence(1). This problem is of fundamental importance as the structure of a protein largely determines its function(2)  however, protein structures can be difficult to determine experimentally. Considerable progress has recently been made by leveraging genetic information. It is possible to infer which amino acid residues are in contact by analysing covariation in homologous sequences, which aids in the prediction of protein structures(3). Here we show that we can train a neural network to make accurate predictions of the distances between pairs of residues, which convey more information about the structure than contact predictions. Using this information, we construct a potential of mean force(4) that can accurately describe the shape of a protein. We find that the resulting potential can be optimized by a simple gradient descent algorithm to generate structures without complex sampling procedures. The resulting system, named AlphaFold, achieves high accuracy, even for sequences with fewer homologous sequences. In the recent Critical Assessment of Protein Structure Prediction(5) (CASP13)-a blind assessment of the state of the field-AlphaFold created high-accuracy structures (with template modelling (TM) scores(6) of 0.7 or higher) for 24 out of 43 free modelling domains, whereas the next best method, which used sampling and contact information, achieved such accuracy for only 14 out of 43 domains. AlphaFold represents a considerable advance in protein-structure prediction. We expect this increased accuracy to enable insights into the function and malfunction of proteins, especially in cases for which no structures for homologous proteins have been experimentally determined(7).


  
Structure of Schlafen13 reveals a new class of tRNA/rRNA- targeting RNase engaged in translational control 期刊论文
NATURE COMMUNICATIONS, 2018, 9
作者:  Yang, Jin-Yu;  Deng, Xiang-Yu;  Li, Yi-Sheng;  Ma, Xian-Cai;  Feng, Jian-Xiong;  Yu, Bing;  Chen, Yang;  Luo, Yi-Ling;  Wang, Xi;  Chen, Mei-Ling;  Fang, Zhi-Xin;  Zheng, Fu-Xiang;  Li, Yi-Ping;  Zhong, Qian;  Kang, Tie-Bang;  Song, Li-Bing;  Xu, Rui-Hua;  Zeng, Mu-Sheng;  Chen, Wei;  Zhang, Hui;  Xie, Wei;  Gao, Song
收藏  |  浏览/下载:10/0  |  提交时间:2019/11/27
Endothelial TLR4 and the microbiome drive cerebral cavernous malformations 期刊论文
NATURE, 2017, 545 (7654) : 305-+
作者:  Tang, Alan T.;  Hoi, Jaesung P. C.;  Kotzin, Jonathan J.;  Yang, Yiqing;  Hong, Courtney C.;  Hobson, Nicholas;  Girard, Romuald;  Zeineddine, Hussein A.;  Lightle, Rhonda;  Moore, Thomas;  Cao, Ying;  Shenkar, Robert;  Chen, Mei;  Mericko, Patricia;  Yang, Jisheng;  Li, Li;  Tanes, Ceylan;  Kobuley, Dmytro;  Vosa, Urmo;  Whitehead, Kevin J.;  Li, Dean Y.;  Franke, Lude;  Hart, Blaine;  Schwaninger, Markus;  Henao-Mejia, Jorge;  Morrison, Leslie;  Kim, Helen;  Awad, Issam A.;  Zheng, Xiangjian;  Kahn, Mark L.
收藏  |  浏览/下载:10/0  |  提交时间:2019/11/27