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Structure and mechanism of human diacylglycerol O-acyltransferase 1 期刊论文
NATURE, 2020, 581 (7808) : 329-+
作者:  Wu, Fan;  Zhao, Su;  Yu, Bin;  Chen, Yan-Mei;  Wang, Wen;  Song, Zhi-Gang;  Hu, Yi;  Tao, Zhao-Wu;  Tian, Jun-Hua;  Pei, Yuan-Yuan;  Yuan, Ming-Li;  Zhang, Yu-Ling;  Dai, Fa-Hui;  Liu, Yi;  Wang, Qi-Min;  Zheng, Jiao-Jiao;  Xu, Lin;  Holmes, Edward C.;  Zhang, Yong-Zhen
收藏  |  浏览/下载:25/0  |  提交时间:2020/07/03

The structure of human diacylglycerol O-acyltransferase 1, a membrane protein that synthesizes triacylglycerides, is solved with cryo-electron microscopy, providing insight into its function and mechanism of enzymatic activity.


Diacylglycerol O-acyltransferase 1 (DGAT1) synthesizes triacylglycerides and is required for dietary fat absorption and fat storage in humans(1). DGAT1 belongs to the membrane-bound O-acyltransferase (MBOAT) superfamily, members of which are found in all kingdoms of life and are involved in the acylation of lipids and proteins(2,3). How human DGAT1 and other mammalian members of the MBOAT family recognize their substrates and catalyse their reactions is unknown. The absence of three-dimensional structures also hampers rational targeting of DGAT1 for therapeutic purposes. Here we present the cryo-electron microscopy structure of human DGAT1 in complex with an oleoyl-CoA substrate. Each DGAT1 protomer has nine transmembrane helices, eight of which form a conserved structural fold that we name the MBOAT fold. The MBOAT fold in DGAT1 forms a hollow chamber in the membrane that encloses highly conserved catalytic residues. The chamber has separate entrances for each of the two substrates, fatty acyl-CoA and diacylglycerol. DGAT1 can exist as either a homodimer or a homotetramer and the two forms have similar enzymatic activity. The N terminus of DGAT1 interacts with the neighbouring protomer and these interactions are required for enzymatic activity.


  
The online competition between pro- and anti-vaccination views 期刊论文
NATURE, 2020, 582 (7811) : 230-+
作者:  Wu, Fan;  Zhao, Su;  Yu, Bin;  Chen, Yan-Mei;  Wang, Wen;  Song, Zhi-Gang;  Hu, Yi;  Tao, Zhao-Wu;  Tian, Jun-Hua;  Pei, Yuan-Yuan;  Yuan, Ming-Li;  Zhang, Yu-Ling;  Dai, Fa-Hui;  Liu, Yi;  Wang, Qi-Min;  Zheng, Jiao-Jiao;  Xu, Lin;  Holmes, Edward C.;  Zhang, Yong-Zhen
收藏  |  浏览/下载:10/0  |  提交时间:2020/07/03

Insights into the interactions between pro- and anti-vaccination clusters on Facebook can enable policies and approaches that attempt to interrupt the shift to anti-vaccination views and persuade undecided individuals to adopt a pro-vaccination stance.


Distrust in scientific expertise(1-14) is dangerous. Opposition to vaccination with a future vaccine against SARS-CoV-2, the causal agent of COVID-19, for example, could amplify outbreaks(2-4), as happened for measles in 2019(5,6). Homemade remedies(7,8) and falsehoods are being shared widely on the Internet, as well as dismissals of expert advice(9-11). There is a lack of understanding about how this distrust evolves at the system level(13,14). Here we provide a map of the contention surrounding vaccines that has emerged from the global pool of around three billion Facebook users. Its core reveals a multi-sided landscape of unprecedented intricacy that involves nearly 100 million individuals partitioned into highly dynamic, interconnected clusters across cities, countries, continents and languages. Although smaller in overall size, anti-vaccination clusters manage to become highly entangled with undecided clusters in the main online network, whereas pro-vaccination clusters are more peripheral. Our theoretical framework reproduces the recent explosive growth in anti-vaccination views, and predicts that these views will dominate in a decade. Insights provided by this framework can inform new policies and approaches to interrupt this shift to negative views. Our results challenge the conventional thinking about undecided individuals in issues of contention surrounding health, shed light on other issues of contention such as climate change(11), and highlight the key role of network cluster dynamics in multi-species ecologies(15).


  
Cascade of Internal Wave Energy Catalyzed by Eddy-Topography Interactions in the Deep South China Sea 期刊论文
GEOPHYSICAL RESEARCH LETTERS, 2020, 47 (4)
作者:  Hu, Qianwen;  Huang, Xiaodong;  Zhang, Zhiwei;  Zhang, Xiaojiang;  Xu, Xing;  Sun, Hui;  Zhou, Chun;  Zhao, Wei;  Tian, Jiwei
收藏  |  浏览/下载:11/0  |  提交时间:2020/07/02
Responses of soil moisture to regional climate change over the Three Rivers Source Region on the Tibetan Plateau 期刊论文
INTERNATIONAL JOURNAL OF CLIMATOLOGY, 2019
作者:  Deng, Mingshan;  Meng, Xianhong;  Li, Zhaoguo;  Lyv, Yaqiong;  Lei, Huajin;  Zhao, Lin;  Zhao, Shengnan;  Ge, Jun;  Jing, Hui
收藏  |  浏览/下载:9/0  |  提交时间:2019/11/27
land surface model data  precipitation  reanalysis data  soil moisture  temperature  Three Rivers Source region  
The Trend Reversal of Dust Aerosol Over East Asia and the North Pacific Ocean Attributed to Large-Scale Meteorology, Deposition, and Soil Moisture 期刊论文
JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES, 2019
作者:  Guo, Jianping;  Xu, Hui;  Liu, Lin;  Chen, Dandan;  Peng, Yiran;  Yim, Steve Hung-Lam;  Yang, Yuanjian;  Li, Jian;  Zhao, Chun;  Zhai, Panmao
收藏  |  浏览/下载:10/0  |  提交时间:2019/11/27
dust  AOD  East Asia  shift  wind  China  
Nitrogen addition effects on tree growth and soil properties mediated by soil phosphorus availability and tree species identity 期刊论文
FOREST ECOLOGY AND MANAGEMENT, 2019, 449
作者:  Zhao, Qiong;  Zeng, De-Hui
收藏  |  浏览/下载:14/0  |  提交时间:2019/11/27
Nitrogen addition  Phosphorus addition  Pious sylvestris var. mongolica  Populus simonii  Soil microbial biomass  Nutrient availability  
Network parameters quantify loss of assemblage structure in human-impacted lake ecosystems 期刊论文
GLOBAL CHANGE BIOLOGY, 2019, 25 (11) : 3871-3882
作者:  Wang, Rong;  Dearing, John A.;  Doncaster, C. Patrick;  Yang, Xiangdong;  Zhang, Enlou;  Langdon, Peter G.;  Yang, Hui;  Dobg, Xuhui;  Hu, Zhujun;  Xu, Min;  Zhao, Yanjie;  Shen, Ji
收藏  |  浏览/下载:6/0  |  提交时间:2019/11/27
biodiversity  diatom assemblages  ecological resilience  network skewness  self-organized system  stability  
Observed Interactions Between Black Carbon and Hydrometeor During Wet Scavenging in Mixed-Phase Clouds 期刊论文
GEOPHYSICAL RESEARCH LETTERS, 2019, 46 (14) : 8453-8463
作者:  Ding, Shuo;  Zhao, Delong;  He, Cenlin;  Huang, Mengyu;  He, Hui;  Tian, Ping;  Liu, Quan;  Bi, Kai;  Yu, Chenjie;  Pitt, Joseph;  Chen, Ng;  Ma, Xincheng;  Chen, Yunbo;  Jia, Xingcan;  Kong, Shaofei;  Wu, Jian;  Hu, Dawei;  Hu, Kang;  Ding, Deping;  Liu, Dantong
收藏  |  浏览/下载:12/0  |  提交时间:2019/11/27
black carbon  wet scavenging  WBF process  aerosol-cloud interaction  
Climate change impacts on Canadian yields of spring wheat, canola and maize for global warming levels of 1.5 degrees C, 2.0 degrees C, 2.5 degrees C and 3.0 degrees C 期刊论文
ENVIRONMENTAL RESEARCH LETTERS, 2019, 14 (7)
作者:  Qian, Budong;  Zhang, Xuebin;  Smith, Ward;  Grant, Brian;  Jing, Qi;  Cannon, Alex J.;  Neilsen, Denise;  McConkey, Brian;  Li, Guilong;  Bonsal, Barrie;  Wan, Hui;  Xue, Li;  Zhao, Jun
收藏  |  浏览/下载:6/0  |  提交时间:2019/11/27
agriculture  Canada  climate change  climate targets  crop yields  crop models  
Modeling Study of the Air Quality Impact of Record-Breaking Southern California Wildfires in December 2017 期刊论文
JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES, 2019, 124 (12) : 6554-6570
作者:  Shi, Hongrong;  Jiang, Zhe;  Zhao, Bin;  Li, Zhijin;  Chen, Yang;  Gu, Yu;  Jiang, Jonathan H.;  Lee, Meemong;  Liou, Kuo-Nan;  Neu, Jessica L.;  Payne, Vivienne H.;  Su, Hui;  Wang, Yuan;  Witek, Marcin;  Worden, John
收藏  |  浏览/下载:10/0  |  提交时间:2019/11/26