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Electromechanical coupling in the hyperpolarization-activated K+ channel KAT1 期刊论文
NATURE, 2020, 583 (7814) : 145-+
作者:  Jin, Zhenming;  Du, Xiaoyu;  Xu, Yechun;  Deng, Yongqiang;  Liu, Meiqin;  Zhao, Yao;  Zhang, Bing;  Li, Xiaofeng;  Zhang, Leike;  Peng, Chao;  Duan, Yinkai;  Yu, Jing;  Wang, Lin;  Yang, Kailin;  Liu, Fengjiang;  Jiang, Rendi;  Yang, Xinglou;  You, Tian;  Liu, Xiaoce
收藏  |  浏览/下载:28/0  |  提交时间:2020/07/03

Voltage-gated potassium (K-v) channels coordinate electrical signalling and control cell volume by gating in response to membrane depolarization or hyperpolarization. However, although voltage-sensing domains transduce transmembrane electric field changes by a common mechanism involving the outward or inward translocation of gating charges(1-3), the general determinants of channel gating polarity remain poorly understood(4). Here we suggest a molecular mechanism for electromechanical coupling and gating polarity in non-domain-swapped K-v channels on the basis of the cryo-electron microscopy structure of KAT1, the hyperpolarization-activated K-v channel from Arabidopsis thaliana. KAT1 displays a depolarized voltage sensor, which interacts with a closed pore domain directly via two interfaces and indirectly via an intercalated phospholipid. Functional evaluation of KAT1 structure-guided mutants at the sensor-pore interfaces suggests a mechanism in which direct interaction between the sensor and the C-linker hairpin in the adjacent pore subunit is the primary determinant of gating polarity. We suggest that an inward motion of the S4 sensor helix of approximately 5-7 angstrom can underlie a direct-coupling mechanism, driving a conformational reorientation of the C-linker and ultimately opening the activation gate formed by the S6 intracellular bundle. This direct-coupling mechanism contrasts with allosteric mechanisms proposed for hyperpolarization-activated cyclic nucleotide-gated channels(5), and may represent an unexpected link between depolarization- and hyperpolarization-activated channels.


The cryo-electron microscopy structure of the hyperpolarization-activated K+ channel KAT1 points to a direct-coupling mechanism between S4 movement and the reorientation of the C-linker.


  
Anthropogenic Aerosols Significantly Reduce Mesoscale Convective System Occurrences and Precipitation Over Southern China in April 期刊论文
GEOPHYSICAL RESEARCH LETTERS, 2020, 47 (6)
作者:  Zhang, Lijuan;  Fu, Tzung-May;  Tian, Heng;  Ma, Yaping;  Chen, Jen-Ping;  Tsai, Tzu-Chin;  Tsai, I-Chun;  Meng, Zhiyong;  Yang, Xin
收藏  |  浏览/下载:15/0  |  提交时间:2020/07/02
anthropogenic aerosols  precipitation  mesoscale convective systems  aerosol-cloud interactions  aerosol-radiation interactions  
Assessing the role of live poultry trade in community-structured transmission of avian influenza in China 期刊论文
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2020, 117 (11) : 5949-5954
作者:  Yang, Qiqi;  Zhao, Xiang;  Lemey, Philippe;  Suchard, Marc A.;  Bi, Yuhai;  Shi, Weifeng;  Liu, Di;  Qi, Wenbao;  Zhang, Guogang;  Stenseth, Nils Chr.;  Pybus, Oliver G.;  Tian, Huaiyu
收藏  |  浏览/下载:12/0  |  提交时间:2020/05/13
avian influenza  poultry trade  phylogeography  community-structured transmission  
Ice front blocking of ocean heat transport to an Antarctic ice shelf 期刊论文
NATURE, 2020, 578 (7796) : 568-+
作者:  Alexandrov, Ludmil B.;  Kim, Jaegil;  Haradhvala, Nicholas J.;  Huang, Mi Ni;  Ng, Alvin Wei Tian;  Wu, Yang;  Boot, Arnoud;  Covington, Kyle R.;  Gordenin, Dmitry A.;  Bergstrom, Erik N.;  Islam, S. M. Ashiqul;  Lopez-Bigas, Nuria;  Klimczak, Leszek J.;  McPherson, John R.;  Morganella, Sandro;  Sabarinathan, Radhakrishnan;  Wheeler, David A.;  Mustonen, Ville;  Getz, Gad;  Rozen, Steven G.;  Stratton, Michael R.
收藏  |  浏览/下载:13/0  |  提交时间:2020/05/13

The front of the Getz Ice Shelf in West Antarctica creates an abrupt topographic step that deflects ocean currents, suppressing 70% of the heat delivery to the ice sheet.


Mass loss from the Antarctic Ice Sheet to the ocean has increased in recent decades, largely because the thinning of its floating ice shelves has allowed the outflow of grounded ice to accelerate(1,2). Enhanced basal melting of the ice shelves is thought to be the ultimate driver of change(2,3), motivating a recent focus on the processes that control ocean heat transport onto and across the seabed of the Antarctic continental shelf towards the ice(4-6). However, the shoreward heat flux typically far exceeds that required to match observed melt rates(2,7,8), suggesting that other critical controls exist. Here we show that the depth-independent (barotropic) component of the heat flow towards an ice shelf is blocked by the marked step shape of the ice front, and that only the depth-varying (baroclinic) component, which is typically much smaller, can enter the sub-ice cavity. Our results arise from direct observations of the Getz Ice Shelf system and laboratory experiments on a rotating platform. A similar blocking of the barotropic component may occur in other areas with comparable ice-bathymetry configurations, which may explain why changes in the density structure of the water column have been found to be a better indicator of basal melt rate variability than the heat transported onto the continental shelf(9). Representing the step topography of the ice front accurately in models is thus important for simulating ocean heat fluxes and induced melt rates.


  
Managing energy infrastructure to decarbonize industrial parks in China 期刊论文
NATURE COMMUNICATIONS, 2020, 11 (1)
作者:  Guo, Yang;  Tian, Jinping;  Chen, Lyujun
收藏  |  浏览/下载:12/0  |  提交时间:2020/05/13
Evolutionary selection of biofilm-mediated extended phenotypes in Yersinia pestis in response to a fluctuating environment 期刊论文
NATURE COMMUNICATIONS, 2020, 11 (1)
作者:  Cui, Yujun;  Schmid, Boris, V;  Cao, Hanli;  Dai, Xiang;  Du, Zongmin;  Easterday, W. Ryan;  Fang, Haihong;  Guo, Chenyi;  Huang, Shanqian;  Liu, Wanbing;  Qi, Zhizhen;  Song, Yajun;  Tian, Huaiyu;  Wang, Min;  Wu, Yarong;  Xu, Bing;  Yang, Chao;  Yang, Jing;  Yang, Xianwei;  Zhang, Qingwen;  Jakobsen, Kjetill S.;  Zhang, Yujiang;  Stenseth, Nils Chr;  Yang, Ruifu
收藏  |  浏览/下载:19/0  |  提交时间:2020/05/13
Field-experiment constraints on the enhancement of the terrestrial carbon sink by CO(2 )fertilization 期刊论文
NATURE GEOSCIENCE, 2019, 12 (10) : 809-+
作者:  Liu, Yongwen;  Piao, Shilong;  Gasser, Thomas;  Ciais, Philippe;  Yang, Hui;  Wang, Han;  Keenan, Trevor F.;  Huang, Mengtian;  Wan, Shiqiang;  Song, Jian;  Wang, Kai;  Janssens, Ivan A.;  Penuelas, Josep;  Huntingford, Chris;  Wang, Xuhui;  Arain, Muhammad Altaf;  Fang, Yuanyuan;  Fisher, Joshua B.;  Huang, Maoyi;  Huntzinger, Deborah N.;  Ito, Akihiko;  Jain, Atul K.;  Mao, Jiafu;  Michalak, Anna M.;  Peng, Changhui;  Poulter, Benjamin;  Schwalm, Christopher;  Shi, Xiaoying;  Tian, Hanqin;  Wei, Yaxing;  Zeng, Ning;  Zhu, Qiuan;  Wang, Tao
收藏  |  浏览/下载:14/0  |  提交时间:2019/11/27
Long-term nitrogen addition modifies microbial composition and functions for slow carbon cycling and increased sequestration in tropical forest soil 期刊论文
GLOBAL CHANGE BIOLOGY, 2019, 25 (10) : 3267-3281
作者:  Tian, Jing;  Dungait, Jennifer A. J.;  Lu, Xiankai;  Yang, Yunfeng;  Hartley, Iain P.;  Zhang, Wei;  Mo, Jiangming;  Yu, Guirui;  Zhou, Jizhong;  Kuzyakov, Yakov
收藏  |  浏览/下载:11/0  |  提交时间:2019/11/27
biogeochemical cycling  C and N turnover  global climate change  microbial functional community  N deposition  tropical forest  
Regulatory mechanism design of GHG emissions in the electric power industry in China 期刊论文
ENERGY POLICY, 2019, 131: 187-201
作者:  Feng, Tian-tian;  Gong, Xiao-lei;  Guo, Yu-hua;  Yang, Yi-sheng;  Dong, Jun
收藏  |  浏览/下载:11/0  |  提交时间:2019/11/27
Electric power industry  Greenhouse gas emission  Regulatory mechanism  
Atmospheric Processing of Loess Particles in a Polluted Urban Area of Northwestern China 期刊论文
JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES, 2019, 124 (14) : 7919-7929
作者:  Chen, Yang;  Liu, Huanwu;  Huang, Ru-Jin;  Yang, Fumo;  Tian, Mi;  Yao, Xiaojiang;  Shen, Zhenxing;  Yan, Lulu;  Cao, Junji
收藏  |  浏览/下载:12/0  |  提交时间:2019/11/27
dust  processing  single particle  urban  aging  loess