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HDAC6 mediates an aggresome-like mechanism for NLRP3 and pyrin inflammasome activation 期刊论文
Science, 2020
作者:  Venkat Giri Magupalli;  Roberto Negro;  Yuzi Tian;  Arthur V. Hauenstein;  Giuseppe Di Caprio;  Wesley Skillern;  Qiufang Deng;  Pontus Orning;  Hasan B. Alam;  Zoltan Maliga;  Humayun Sharif;  Jun Jacob Hu;  Charles L. Evavold;  Jonathan C. Kagan;  Florian I. Schmidt;  Katherine A. Fitzgerald;  Tom Kirchhausen;  Yongqing Li;  Hao Wu
收藏  |  浏览/下载:9/0  |  提交时间:2020/09/22
The hepatocyte clock and feeding control chronophysiology of multiple liver cell types 期刊论文
Science, 2020
作者:  Dongyin Guan;  Ying Xiong;  Trang Minh Trinh;  Yang Xiao;  Wenxiang Hu;  Chunjie Jiang;  Pieterjan Dierickx;  Cholsoon Jang;  Joshua D. Rabinowitz;  Mitchell A. Lazar
收藏  |  浏览/下载:7/0  |  提交时间:2020/09/14
A less cloudy picture of the inter-model spread in future global warming projections 期刊论文
Nature Communications, 2020
作者:  Xiaoming Hu;  Hanjie Fan;  Ming Cai;  Sergio A. Sejas;  Patrick Taylor;  Song Yang
收藏  |  浏览/下载:5/0  |  提交时间:2020/09/14
Number of HIV-1 founder variants is determined by the recency of the source partner infection 期刊论文
Science, 2020
作者:  Ch. Julián Villabona-Arenas;  Matthew Hall;  Katrina A. Lythgoe;  Stephen G. Gaffney;  Roland R. Regoes;  Stéphane Hué;  Katherine E. Atkins
收藏  |  浏览/下载:7/0  |  提交时间:2020/07/06
Gas chromatography–mass spectrometry analyses of encapsulated stable perovskite solar cells 期刊论文
Science, 2020
作者:  Lei Shi;  Martin P. Bucknall;  Trevor L. Young;  Meng Zhang;  Long Hu;  Jueming Bing;  Da Seul Lee;  Jincheol Kim;  Tom Wu;  Noboru Takamure;  David R. McKenzie;  Shujuan Huang;  Martin A. Green;  Anita W. Y. Ho-Baillie
收藏  |  浏览/下载:7/0  |  提交时间:2020/06/22
Structures of human pannexin 1 reveal ion pathways and mechanism of gating 期刊论文
NATURE, 2020
作者:  Krause, David W.;  Hoffmann, Simone;  Hu, Yaoming;  Wible, John R.;  Rougier, Guillermo W.;  Kirk, E. Christopher;  Groenke, Joseph R.;  Rogers, Raymond R.;  Rossie, James B.;  Schultz, Julia A.;  Evans, Alistair R.;  von Koenigswald, Wighart;  Rahantarisoa, Lydia J.
收藏  |  浏览/下载:9/0  |  提交时间:2020/07/03

Cryo-electron microscopy structures of the ATP-permeable channel pannexin 1 reveal a gating mechanism involving multiple distinct ion-conducting pathways.


Pannexin 1 (PANX1) is an ATP-permeable channel with critical roles in a variety of physiological functions such as blood pressure regulation(1), apoptotic cell clearance(2) and human oocyte development(3). Here we present several structures of human PANX1 in a heptameric assembly at resolutions of up to 2.8 angstrom, including an apo state, a caspase-7-cleaved state and a carbenoxolone-bound state. We reveal a gating mechanism that involves two ion-conducting pathways. Under normal cellular conditions, the intracellular entry of the wide main pore is physically plugged by the C-terminal tail. Small anions are conducted through narrow tunnels in the intracellular domain. These tunnels connect to the main pore and are gated by a long linker between the N-terminal helix and the first transmembrane helix. During apoptosis, the C-terminal tail is cleaved by caspase, allowing the release of ATP through the main pore. We identified a carbenoxolone-binding site embraced by W74 in the extracellular entrance and a role for carbenoxolone as a channel blocker. We identified a gap-junction-like structure using a glycosylation-deficient mutant, N255A. Our studies provide a solid foundation for understanding the molecular mechanisms underlying the channel gating and inhibition of PANX1 and related large-pore channels.


  
Observation of the Kondo screening cloud 期刊论文
NATURE, 2020, 579 (7798) : 210-+
作者:  Shahnawaz, Mohammad;  Mukherjee, Abhisek;  Pritzkow, Sandra;  Mendez, Nicolas;  Rabadia, Prakruti;  Liu, Xiangan;  Hu, Bo;  Schmeichel, Ann;  Singer, Wolfgang;  Wu, Gang;  Tsai, Ah-Lim;  Shirani, Hamid;  Nilsson, K. Peter R.;  Low, Phillip A.;  Soto, Claudio
收藏  |  浏览/下载:11/0  |  提交时间:2020/07/03

When a magnetic impurity exists in a metal, conduction electrons form a spin cloud that screens the impurity spin. This basic phenomenon is called the Kondo effect(1,2). Unlike electric-charge screening, the spin-screening cloud(3-6) occurs quantum coherently, forming spin-singlet entanglement with the impurity. Although the spins interact locally around the impurity, the Kondo cloud can theoretically spread out over several micrometres. The cloud has not so far been detected, and so its physical existence-a fundamental aspect of the Kondo effect-remains controversial(7,8). Here we present experimental evidence of a Kondo cloud extending over a length of micrometres, comparable to the theoretical length xi(K). In our device, a Kondo impurity is formed in a quantum dot(2,9-11), coupling on one side to a quasi-one-dimensional channel(12) that houses a Fabry-Perot interferometer of various gate-defined lengths L exceeding one micrometre. When we sweep a voltage on the interferometer end gate-separated by L from the quantum dot-to induce Fabry-Perot oscillations in conductance we observe oscillations in the measured Kondo temperature T-K, which is a signature of the Kondo cloud at distance L. When L is less than xi(K) the T-K oscillation amplitude becomes larger as L becomes smaller, obeying a scaling function of a single parameter L/xi(K), whereas when L is greater than xi(K) the oscillation is much weaker. Our results reveal that xi(K) is the only length parameter associated with the Kondo effect, and that the cloud lies mostly within a length of xi(K). Our experimental method offers a way of detecting the spatial distribution of exotic non-Fermi liquids formed by multiple magnetic impurities or multiple screening channels(13-16) and of studying spin-correlated systems.


  
Spatiotemporal Variability and Sources of DIC in Permafrost Catchments of the Yangtze River Source Region: Insights From Stable Carbon Isotope and Water Chemistry 期刊论文
WATER RESOURCES RESEARCH, 2020, 56 (1)
作者:  Song, Chunlin;  Wang, Genxu;  Mao, Tianxu;  Huang, Kewei;  Sun, Xiangyang;  Hu, Zhaoyong;  Chang, Ruiying;  Chen, Xiaopeng;  Raymond, Peter A.
收藏  |  浏览/下载:3/0  |  提交时间:2020/07/02