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Structure and mechanism of human diacylglycerol O-acyltransferase 1 期刊论文
NATURE, 2020, 581 (7808) : 329-+
作者:  Wu, Fan;  Zhao, Su;  Yu, Bin;  Chen, Yan-Mei;  Wang, Wen;  Song, Zhi-Gang;  Hu, Yi;  Tao, Zhao-Wu;  Tian, Jun-Hua;  Pei, Yuan-Yuan;  Yuan, Ming-Li;  Zhang, Yu-Ling;  Dai, Fa-Hui;  Liu, Yi;  Wang, Qi-Min;  Zheng, Jiao-Jiao;  Xu, Lin;  Holmes, Edward C.;  Zhang, Yong-Zhen
收藏  |  浏览/下载:24/0  |  提交时间:2020/07/03

The structure of human diacylglycerol O-acyltransferase 1, a membrane protein that synthesizes triacylglycerides, is solved with cryo-electron microscopy, providing insight into its function and mechanism of enzymatic activity.


Diacylglycerol O-acyltransferase 1 (DGAT1) synthesizes triacylglycerides and is required for dietary fat absorption and fat storage in humans(1). DGAT1 belongs to the membrane-bound O-acyltransferase (MBOAT) superfamily, members of which are found in all kingdoms of life and are involved in the acylation of lipids and proteins(2,3). How human DGAT1 and other mammalian members of the MBOAT family recognize their substrates and catalyse their reactions is unknown. The absence of three-dimensional structures also hampers rational targeting of DGAT1 for therapeutic purposes. Here we present the cryo-electron microscopy structure of human DGAT1 in complex with an oleoyl-CoA substrate. Each DGAT1 protomer has nine transmembrane helices, eight of which form a conserved structural fold that we name the MBOAT fold. The MBOAT fold in DGAT1 forms a hollow chamber in the membrane that encloses highly conserved catalytic residues. The chamber has separate entrances for each of the two substrates, fatty acyl-CoA and diacylglycerol. DGAT1 can exist as either a homodimer or a homotetramer and the two forms have similar enzymatic activity. The N terminus of DGAT1 interacts with the neighbouring protomer and these interactions are required for enzymatic activity.


  
The online competition between pro- and anti-vaccination views 期刊论文
NATURE, 2020, 582 (7811) : 230-+
作者:  Wu, Fan;  Zhao, Su;  Yu, Bin;  Chen, Yan-Mei;  Wang, Wen;  Song, Zhi-Gang;  Hu, Yi;  Tao, Zhao-Wu;  Tian, Jun-Hua;  Pei, Yuan-Yuan;  Yuan, Ming-Li;  Zhang, Yu-Ling;  Dai, Fa-Hui;  Liu, Yi;  Wang, Qi-Min;  Zheng, Jiao-Jiao;  Xu, Lin;  Holmes, Edward C.;  Zhang, Yong-Zhen
收藏  |  浏览/下载:10/0  |  提交时间:2020/07/03

Insights into the interactions between pro- and anti-vaccination clusters on Facebook can enable policies and approaches that attempt to interrupt the shift to anti-vaccination views and persuade undecided individuals to adopt a pro-vaccination stance.


Distrust in scientific expertise(1-14) is dangerous. Opposition to vaccination with a future vaccine against SARS-CoV-2, the causal agent of COVID-19, for example, could amplify outbreaks(2-4), as happened for measles in 2019(5,6). Homemade remedies(7,8) and falsehoods are being shared widely on the Internet, as well as dismissals of expert advice(9-11). There is a lack of understanding about how this distrust evolves at the system level(13,14). Here we provide a map of the contention surrounding vaccines that has emerged from the global pool of around three billion Facebook users. Its core reveals a multi-sided landscape of unprecedented intricacy that involves nearly 100 million individuals partitioned into highly dynamic, interconnected clusters across cities, countries, continents and languages. Although smaller in overall size, anti-vaccination clusters manage to become highly entangled with undecided clusters in the main online network, whereas pro-vaccination clusters are more peripheral. Our theoretical framework reproduces the recent explosive growth in anti-vaccination views, and predicts that these views will dominate in a decade. Insights provided by this framework can inform new policies and approaches to interrupt this shift to negative views. Our results challenge the conventional thinking about undecided individuals in issues of contention surrounding health, shed light on other issues of contention such as climate change(11), and highlight the key role of network cluster dynamics in multi-species ecologies(15).


  
Understanding the Impact of Observation Data Uncertainty on Probabilistic Streamflow Forecasts Using a Dynamic Hierarchical Model 期刊论文
WATER RESOURCES RESEARCH, 2020, 56 (4)
作者:  Das Bhowmik, Rajarshi;  Ng, Tze Ling;  Wang, Jui-Pin
收藏  |  浏览/下载:8/0  |  提交时间:2020/07/02
uncertainty  measurement error  Bayesian  BDHM  forecasting  Streamflow  
Assessment of the short-term mortality effect of the national action plan on air pollution in Beijing, China 期刊论文
ENVIRONMENTAL RESEARCH LETTERS, 2020, 15 (3)
作者:  Han, Ling;  Sun, Zhaobin;  Gong, Tianyi;  Zhang, Xiaoling;  He, Juan;  Xing, Qian;  Li, Ziming;  Wang, Ji;  Ye, Dianxiu;  Miao, Shiguang
收藏  |  浏览/下载:6/0  |  提交时间:2020/07/02
air pollution prevention and control action plan  PM2  5  mortality  air quality  emission reduction  
C-14-Dating Model for Groundwater Affected by CO2 Inputs From Deep Underground Formations 期刊论文
WATER RESOURCES RESEARCH, 2020, 56 (3)
作者:  Wang, Tao;  Chen, Jiansheng;  Zhang, Chenming;  Zhan, Lucheng;  Li, Ling
收藏  |  浏览/下载:9/0  |  提交时间:2020/07/02
Fog Interception Maintains a Major Waterfall Landscape in Southwest China Revealed by Isotopic Signatures 期刊论文
WATER RESOURCES RESEARCH, 2020, 56 (3)
作者:  Zhan, Lucheng;  Chen, Jiansheng;  Zhang, Chenming;  Wang, Tao;  Xin, Pei;  Li, Ling
收藏  |  浏览/下载:17/0  |  提交时间:2020/07/02
Atmospheric Sulfuric Acid-Dimethylamine Nucleation Enhanced by Trifluoroacetic Acid 期刊论文
GEOPHYSICAL RESEARCH LETTERS, 2020, 47 (2)
作者:  Lu, Yiqun;  Liu, Ling;  Ning, An;  Yang, Gan;  Liu, Yiliang;  Kurten, Theo;  Vehkamaki, Hanna;  Zhang, Xiuhui;  Wang, Lin
收藏  |  浏览/下载:8/0  |  提交时间:2020/07/02
nucleation  new particle formation  cluster  sulfuric acid  dimethylamine  trifluoroacetic acid  
Multi-model analysis of climate impacts on plant photosynthesis in China during 2000-2015 期刊论文
INTERNATIONAL JOURNAL OF CLIMATOLOGY, 2019, 39 (15) : 5539-5555
作者:  Yan, Hao;  Wang, Shao-Qiang;  Cao, Yun;  Xu, Ling-Ling;  Wu, Men-xin;  Cheng, Lu;  Mao, Liu-Xi;  Zhang, Xian-Zhou;  Liu, Yun-Fen;  Wang, Yan-Fen;  Chen, Shi-Ping;  Li, Ying-Nian;  Han, Shi-Jie;  Zhou, Guo-Yi;  Zhang, Yi-Ping;  Shugart, Herman H.;  Wang, Jun-Bang;  Zhao, Feng-Hua
收藏  |  浏览/下载:13/0  |  提交时间:2020/02/17
climate change  dry/wet climate  Gross primary production  meteorological factors  multi-model analysis  
Sulfate formation during heavy winter haze events and the potential contribution from heterogeneous SO2 + NO2 reactions in the Yangtze River Delta region, China 期刊论文
ATMOSPHERIC CHEMISTRY AND PHYSICS, 2019, 19 (22) : 14311-14328
作者:  Huang, Ling;  An, Jingyu;  Koo, Bonyoung;  Yarwood, Greg;  Yan, Rusha;  Wang, Yangjun;  Huang, Cheng;  Li, Li
收藏  |  浏览/下载:8/0  |  提交时间:2020/02/17
A genome-wide association study identifies six novel risk loci for primary biliary cholangitis 期刊论文
NATURE COMMUNICATIONS, 2017, 8
作者:  Qiu, Fang;  Tang, Ruqi;  Zuo, Xianbo;  Shi, Xingjuan;  Wei, Yiran;  Zheng, Xiaodong;  Dai, Yaping;  Gong, Yuhua;  Wang, Lan;  Xu, Ping;  Zhu, Xiang;  Wu, Jian;  Han, Chongxu;  Gao, Yueqiu;  Zhang, Kui;  Jiang, Yuzhang;  Zhou, Jianbo;  Shao, Youlin;  Hu, Zhigang;  Tian, Ye;  Zhang, Haiyan;  Dai, Na;  Liu, Lei;  Wu, Xudong;  Zhao, Weifeng;  Zhang, Xiaomin;  Zang, Zhidong;  Nie, Jinshan;  Sun, Weihao;  Zhao, Yi;  Mao, Yuan;  Jiang, Po;  Ji, Hualiang;  Dong, Qing;  Li, Junming;  Li, Zhenzhong;  Bai, Xinli;  Li, Li;  Lin, Maosong;  Dong, Ming;  Li, Jinxin;  Zhu, Ping;  Wang, Chan;  Zhang, Yanqiu;  Jiang, Peng;  Wang, Yujue;  Jawed, Rohil;  Xu, Jing;  Zhang, Yu;  Wang, Qixia;  Yang, Yue;  Yang, Fan;  Lian, Min;  Jiang, Xiang;  Xiao, Xiao;  Li, Yanmei;  Fang, Jingyuan;  Qiu, Dekai;  Zhu, Zhen;  Qiu, Hong;  Zhang, Jianqiong;  Tian, Wenyan;  Chen, Sufang;  Jiang, Ling;  Ji, Bing;  Li, Ping;  Chen, Guochang;  Wu, Tianxue;  Sun, Yan;  Yu, Jianjiang;  Tang, Huijun;  He, Michun;  Xia, Min;  Pei, Hao;  Huang, Lihua;  Qing, Zhuye;  Wu, Jianfang;  Huang, Qinghai;  Han, Junhai;  Xie, Wei;  Sun, Zhongsheng;  Guo, Jian;  He, Gengsheng;  Gershwin, M. Eric;  Lian, Zhexiong;  Liu, Xiang;  Seldin, Michael F.;  Liu, Xiangdong;  Chen, Weichang;  Ma, Xiong
收藏  |  浏览/下载:17/0  |  提交时间:2019/11/27