LinkPI: Linking Phenotype function with Identity: a novel integrated single-cell technology and metagenomics approach

GSTDTAP

项目编号	NE/S008721/1
	LinkPI: Linking Phenotype function with Identity: a novel integrated single-cell technology and metagenomics approach
	Huabing Yin
主持机构	University of Glasgow
项目开始年	2018
	2018-09-01
项目结束日期	2020-08-31
资助机构	UK-NERC
项目类别	Research Grant
项目经费	83395(GBP)
国家	英国
语种	英语
英文摘要	The wide ranging and diverse microorganisms found within the environment play a central role in maintaining sustainability on our planet. However, their ability to function and the functions themselves have been and are being seriously altered by human activities. One key example of this is the development of antimicrobial resistance in wild bacteria as a result of antibiotics that can be found in waste water. The impact of this is a significant emerging threat to the global economy and health. Increasing evidence shows that variations in the genetic makeup of individual cells, together with the way these are manifest in their physical characteristics plays a critical role in the fate of these microorganisms. Despite this knowledge, most studies of microorganisms currently rely on culturing and analysing them as large groups in laboratories, rather than on the individual level. To further complicate matters, the majority of the naturally occurring microbes (>99%) are not capable of being grown in laboratory conditions. This imposes formidable challenges to understand the activities of microbes in situ and their response to the ever-changing environments. In this project we will establish a novel approach to identify active microbes of interest within complex microbial communities, linking the behaviour and genetic profile of individual cells. Specifically, we will exploit the cutting-edge technology advances to analyse, sort and characterise microbes from a mixture of microorganisms. To achieve this, we will work to develop new devices and protocols for collecting samples on-site. These samples will then be analysed using a range of cutting-edge, lab-based techniques. One key feature of the project is the forging of new international collaborations with the world leading research groups of complementary expertise. This not only provides us with access to a range of world-class tools, but also enables the local collection and handling of samples from sites of interest around the globe. These include the Yanzi river near Shanghai in China, the River Thames near London and the Han River near Seoul in Korea.
来源学科分类	Natural Environment Research
文献类型	项目
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/87341
专题	环境与发展全球科技态势
推荐引用方式 GB/T 7714	Huabing Yin.LinkPI: Linking Phenotype function with Identity: a novel integrated single-cell technology and metagenomics approach.2018.