Occurrence and horizontal gene transfer of carbapenemase and ESBL genes in soil microbiomes

GSTDTAP

项目编号	NE/N02026X/1
	Occurrence and horizontal gene transfer of carbapenemase and ESBL genes in soil microbiomes
	[unavailable]
主持机构	University of Southampton
项目开始年	2016
	2016-06-01
项目结束日期	2018-02-28
资助机构	UK-NERC
项目类别	Research Grant
国家	英国
语种	英语
英文摘要	This project aims to understand the potential of environmental reservoirs and transmission of AMR in members of autochthonous soil microbiomes and between transient allochthonous human and animal pathogens entering the environment, for example from faecal agricultural wastes or domesticated or wild animal and bird faecal ingress. It also seeks to understand if horizontal gene transfer occurs successfully between potential pathogens in the various soil microbiomes and complex matrices encompassing clay, loam and sandy soils. The work will utilize modern molecular biology and genomics to screen microbiome populations and defined third generation ESBL and fourth generation carbapenemase E. coli and Klebsiella pneumoniae donor strains, with an E. coli sensitive strain as the recipient. The University of Southampton team comprises an established scientific group with expertise in survival of faecal pathogens in soil and water, and HGT of ESBL and carbapenemase resistance genes between different species, in collaboration with an Early Career Researcher with expertise in soil microbiology and processes, and stable isotope probing (SIP) to understand community viability, metabolic turnover and gene acquisition. Previous work for UKWIR and Defra/FSA has developed grassed soil microcosms of clay, loam and sandy soils and demonstrated survival of zoonotic pathogens therein for several weeks following faecal waste irrigation. These pathogens were subsequently released from the complex soil matrices using a novel, gentle pulsification procedure followed by membrane filtration and quantitative resuscitation on appropriate selective agar media. This proposal will help identify the specific environmental drivers of the HGT and beta lactamase selection processes, including implications for both anthropogenic (animal husbandry, human wastewater disposal) and non-anthropogenic (wild animal and bird faecal ingress) drivers. The work will also include identifying the implications for pathogens of clinical and/or veterinary importance such Klebsiella pneumonia and E. coli. The research will be able to help inform AMR policy and management strategies, and will form the basis of a subsequent large research proposal to address these policies and strategies more fully. The approaches and methodologies to be developed in this proposal could be translated to other environments, antibacterials or other bacterial communities of interest to the AMR funding bodies.
来源学科分类	Natural Environment Research
文献类型	项目
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/86209
专题	环境与发展全球科技态势
推荐引用方式 GB/T 7714	[unavailable].Occurrence and horizontal gene transfer of carbapenemase and ESBL genes in soil microbiomes.2016.