Engineering synthetic morphogen systems that can program multicellular patterning

doi:10.1126/science.abc0033

GSTDTAP > 气候变化

DOI	10.1126/science.abc0033
	Engineering synthetic morphogen systems that can program multicellular patterning
	Satoshi Toda; Wesley L. McKeithan; Teemu J. Hakkinen; Pilar Lopez; Ophir D. Klein; Wendell A. Lim
	2020-10-16
发表期刊	Science
出版年	2020
英文摘要	Morphogens provide positional information during tissue development. For this behavior to occur, morphogens must spread out and form a concentration gradient; however, their mechanism of transport remains a matter of debate. Stapornwongkul et al. now show that in the presence of extracellular binding elements (binders), the inert green fluorescent protein (GFP) can form a detectable concentration gradient by diffusion in the developing fly wing (see the Perspective by Barkai and Shilo). When combining the expression of nonsignaling binders and receptors engineered to respond to GFP, a synthetic GFP gradient can substitute for a natural morphogen to organize growth and patterning. In related work, Toda et al. also show that GFP can be converted into a morphogen by providing anchoring interactions that tether the molecule, forming a gradient that can be recognized by synthetic receptors that activate gene expression. These synthetic morphogens can be used to program de novo multidomain tissue patterns. These results highlight core mechanisms of morphogen signaling and patterning and provide ways to program spatial tissue organization independently from endogenous morphogen pathways. Science , this issue p. [321][1], p. [327][2]; see also p. [292][3] In metazoan tissues, cells decide their fates by sensing positional information provided by specialized morphogen proteins. To explore what features are sufficient for positional encoding, we asked whether arbitrary molecules (e.g., green fluorescent protein or mCherry) could be converted into synthetic morphogens. Synthetic morphogens expressed from a localized source formed a gradient when trapped by surface-anchoring proteins, and they could be sensed by synthetic receptors. Despite their simplicity, these morphogen systems yielded patterns reminiscent of those observed in vivo. Gradients could be reshaped by altering anchor density or by providing a source of competing inhibitor. Gradient interpretation could be altered by adding feedback loops or morphogen cascades to receiver cell response circuits. Orthogonal cell-cell communication systems provide insight into morphogen evolution and a platform for engineering tissues. [1]: /lookup/doi/10.1126/science.abb8205 [2]: /lookup/doi/10.1126/science.abc0033 [3]: /lookup/doi/10.1126/science.abe4217
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/299341
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Satoshi Toda,Wesley L. McKeithan,Teemu J. Hakkinen,et al. Engineering synthetic morphogen systems that can program multicellular patterning[J]. Science,2020.
APA	Satoshi Toda,Wesley L. McKeithan,Teemu J. Hakkinen,Pilar Lopez,Ophir D. Klein,&Wendell A. Lim.(2020).Engineering synthetic morphogen systems that can program multicellular patterning.Science.
MLA	Satoshi Toda,et al."Engineering synthetic morphogen systems that can program multicellular patterning".Science (2020).