Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1073/pnas.1821233116 |
NADPH-dependent extracellular superoxide production is vital to photophysiology in the marine diatom Thalassiosira oceanica | |
Diaz, Julia M.1,2,4; Plummer, Sydney1; Hansel, Colleen M.2; Andeer, Peter F.2,3; Saito, Mak A.2; McIlvin, Matthew R.2 | |
2019 | |
发表期刊 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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ISSN | 0027-8424 |
出版年 | 2019 |
卷号 | 116期号:33页码:16448-16453 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Reactive oxygen species (ROS) like superoxide drive rapid transformations of carbon and metals in aquatic systems and play dynamic roles in biological health, signaling, and defense across a diversity of cell types. In phytoplankton, however, the ecophysiological role(s) of extracellular superoxide production has remained elusive. Here, the mechanism and function of extracellular superoxide production by the marine diatom Thalassiosira oceanica are described. Extracellular superoxide production in T. oceanica exudates was coupled to the oxidation of NADPH. A putative NADPH-oxidizing flavoenzyme with predicted transmembrane domains and high sequence similarity to glutathione reductase (GR) was implicated in this process. GR was also linked to extracellular superoxide production by whole cells via quenching by the flavoenzyme inhibitor diphenylene iodonium (DPI) and oxidized glutathione, the preferred electron acceptor of GR. Extracellular superoxide production followed a typical photosynthesis-irradiance curve and increased by 30% above the saturation irradiance of photosynthesis, while DPI significantly impaired the efficiency of photosystem II under a wide range of light levels. Together, these results suggest that extracellular superoxide production is a byproduct of a transplasma membrane electron transport system that serves to balance the cellular redox state through the recycling of photosynthetic NADPH. This photo-protective function may be widespread, consistent with the presence of putative homologs to T. oceanica GR in other representative marine phytoplankton and ocean metagenomes. Given predicted climate-driven shifts in global surface ocean light regimes and phytoplankton community-level photoacclimation, these results provide implications for future ocean redox balance, ecological functioning, and coupled biogeochemical transformations of carbon and metals. |
英文关键词 | reactive oxygen species photosynthesis oxidative stress biogeochemistry |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000481404300049 |
WOS关键词 | PHYTOPLANKTON CHATTONELLA-MARINA ; HYDROGEN-PEROXIDE ; REDOX ; OXIDATION ; ANION ; IRON ; PHOTOSYNTHESIS ; MECHANISMS ; GENERATION ; REDUCTION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/205160 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Georgia, Dept Marine Sci, Skidaway Inst Oceanog, Savannah, GA 31411 USA; 2.Woods Hole Oceanog Inst, Dept Marine Chem & Geochem, Woods Hole, MA 02543 USA; 3.Lawrence Berkeley Natl Lab, Environm Genom & Syst Biol, Berkeley, CA 94720 USA; 4.Univ Calif San Diego, Scripps Inst Oceanog, Geosci Res Div, La Jolla, CA 92093 USA |
推荐引用方式 GB/T 7714 | Diaz, Julia M.,Plummer, Sydney,Hansel, Colleen M.,et al. NADPH-dependent extracellular superoxide production is vital to photophysiology in the marine diatom Thalassiosira oceanica[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2019,116(33):16448-16453. |
APA | Diaz, Julia M.,Plummer, Sydney,Hansel, Colleen M.,Andeer, Peter F.,Saito, Mak A.,&McIlvin, Matthew R..(2019).NADPH-dependent extracellular superoxide production is vital to photophysiology in the marine diatom Thalassiosira oceanica.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,116(33),16448-16453. |
MLA | Diaz, Julia M.,et al."NADPH-dependent extracellular superoxide production is vital to photophysiology in the marine diatom Thalassiosira oceanica".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 116.33(2019):16448-16453. |
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