Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-019-12100-0 |
Comprehensive correlation analysis for super-resolution dynamic fingerprinting of cellular compartments using the Zeiss Airyscan detector | |
Scipioni, L.1; Lanzano, L.2; Diaspro, A.2,3; Gratton, E.1 | |
2019-09-13 | |
发表期刊 | NATURE COMMUNICATIONS
![]() |
ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Italy |
英文摘要 | The availability of the Airyscan detector in the Zeiss LSM 880 has made possible the development of a new concept in fluctuation correlation spectroscopy using super-resolution. The Airyscan unit acquires data simultaneously on 32 detectors arranged in a hexagonal array. This detector opens up the possibility to use fluctuation methods based on time correlation at single points or at a number of points simultaneously, as well as methods based on spatial correlation in the area covered by the detector. Given the frame rate of this detector, millions of frames can be acquired in seconds, providing a robust statistical basis for fluctuation data. We apply the comprehensive analysis to the molecular fluctuations of free GFP diffusing in live cells at different subcellular compartments to show that at the nanoscale different cell environments can be distinguished by the comprehensive fluctuation analysis. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000451742700025 |
WOS关键词 | FLUORESCENCE CORRELATION SPECTROSCOPY ; PAIR CORRELATION ; DIFFUSION LAWS ; MEMBRANE ; NUMBER ; MICROENVIRONMENT ; BRIGHTNESS ; REVEALS ; CELLS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204539 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Calif Irvine, Dept Biomed Engn, Lab Fluorescence Dynam, Irvine, CA 92697 USA; 2.Ist Italiano Tecnol, Nanoscopy, I-16163 Genoa, Italy; 3.Ist Italiano Tecnol, Nikon Imaging Ctr, I-16163 Genoa, Italy |
推荐引用方式 GB/T 7714 | Scipioni, L.,Lanzano, L.,Diaspro, A.,et al. Comprehensive correlation analysis for super-resolution dynamic fingerprinting of cellular compartments using the Zeiss Airyscan detector[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Scipioni, L.,Lanzano, L.,Diaspro, A.,&Gratton, E..(2019).Comprehensive correlation analysis for super-resolution dynamic fingerprinting of cellular compartments using the Zeiss Airyscan detector.NATURE COMMUNICATIONS,9. |
MLA | Scipioni, L.,et al."Comprehensive correlation analysis for super-resolution dynamic fingerprinting of cellular compartments using the Zeiss Airyscan detector".NATURE COMMUNICATIONS 9(2019). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论