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DOI | 10.1038/s41467-019-11213-w |
Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria | |
Dong, Chen1; Fontana, Jason2; Patel, Anika1; Carothers, James M.2,3,4; Zalatan, Jesse G.1,2,4 | |
2019-09-10 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Methods to regulate gene expression programs in bacterial cells are limited by the absence of effective gene activators. To address this challenge, we have developed synthetic bacterial transcriptional activators in E. coli by linking activation domains to programmable CRISPR-Cas DNA binding domains. Effective gene activation requires target sites situated in a narrow region just upstream of the transcription start site, in sharp contrast to the relatively flexible target site requirements for gene activation in eukaryotic cells. Together with existing tools for CRISPRi gene repression, these bacterial activators enable programmable control over multiple genes with simultaneous activation and repression. Further, the entire gene expression program can be switched on by inducing expression of the CRISPR-Cas system. This work will provide a foundation for engineering synthetic bacterial cellular devices with applications including diagnostics, therapeutics, and industrial biosynthesis. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000436540700007 |
WOS关键词 | ESCHERICHIA-COLI ZWF ; DNA-BINDING ; RNA-POLYMERASE ; CRYSTAL-STRUCTURE ; ETHANOL-PRODUCTION ; BETA-SUBUNIT ; SOXS ; EXPRESSION ; PROTEIN ; SYSTEM |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204532 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Washington, Dept Chem, Seattle, WA 98195 USA; 2.Univ Washington, Mol Engn & Sci Inst, Seattle, WA 98195 USA; 3.Univ Washington, Dept Chem Engn, Seattle, WA 98195 USA; 4.Univ Washington, Ctr Synthet Biol, Seattle, WA 98195 USA |
推荐引用方式 GB/T 7714 | Dong, Chen,Fontana, Jason,Patel, Anika,et al. Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Dong, Chen,Fontana, Jason,Patel, Anika,Carothers, James M.,&Zalatan, Jesse G..(2019).Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria.NATURE COMMUNICATIONS,9. |
MLA | Dong, Chen,et al."Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria".NATURE COMMUNICATIONS 9(2019). |
条目包含的文件 | 条目无相关文件。 |
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