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DOI | 10.1038/s41467-019-11421-4 |
DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle | |
Mikheikin, Andrey1; Olsen, Anita1; Leslie, Kevin1; Russell-Pavier, Freddie2,3; Yacoot, Andrew2; Picco, Loren3; Payton, Oliver3; Toor, Amir4,5; Chesney, Alden5,6; Gimzewski, James K.7; Mishra, Bud8,9; Reed, Jason1,5 | |
2019-08-01 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; England |
英文摘要 | Progress in whole-genome sequencing using short-read (e.g., <150 bp), next-generation sequencing technologies has reinvigorated interest in high-resolution physical mapping to fill technical gaps that are not well addressed by sequencing. Here, we report two technical advances in DNA nanotechnology and single-molecule genomics: (1) we describe a labeling technique (CRISPR-Cas9 nanoparticles) for high-speed AFM-based physical mapping of DNA and (2) the first successful demonstration of using DVD optics to image DNA molecules with high-speed AFM. As a proof of principle, we used this new "nanomapping" method to detect and map precisely BCL2-IGH translocations present in lymph node biopsies of follicular lymphoma patents. This HS-AFM "nanomapping" technique can be complementary to both sequencing and other physical mapping approaches. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000416039500001 |
WOS关键词 | ATOMIC-FORCE MICROSCOPY ; FOLLICULAR LYMPHOMA ; GENOMIC DNA ; BRCA1 ; MICA ; BREAST ; CANCER ; GRADE ; GENE ; PCR |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204479 |
专题 | 资源环境科学 |
作者单位 | 1.Virginia Commonwealth Univ, Dept Phys, Richmond, VA 23284 USA; 2.Natl Phys Lab, Hampton Rd, Teddington TW11 0LW, Middx, England; 3.Interface Anal Ctr, HH Wills Phys Lab, Tyndall Ave, Bristol BS8 1TL, Avon, England; 4.VCU Sch Med, Dept Internal Med, Richmond, VA 23284 USA; 5.VCU Massey Canc Ctr, Richmond, VA 23284 USA; 6.VCU Sch Med, Dept Pathol, Richmond, VA 23284 USA; 7.Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA; 8.NYU, Courant Inst Math Sci, Dept Comp Sci, New York, NY 10012 USA; 9.NYU, Courant Inst Math Sci, Dept Math, New York, NY 10012 USA |
推荐引用方式 GB/T 7714 | Mikheikin, Andrey,Olsen, Anita,Leslie, Kevin,et al. DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle[J]. NATURE COMMUNICATIONS,2019,8. |
APA | Mikheikin, Andrey.,Olsen, Anita.,Leslie, Kevin.,Russell-Pavier, Freddie.,Yacoot, Andrew.,...&Reed, Jason.(2019).DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle.NATURE COMMUNICATIONS,8. |
MLA | Mikheikin, Andrey,et al."DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle".NATURE COMMUNICATIONS 8(2019). |
条目包含的文件 | 条目无相关文件。 |
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