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DOI | 10.1038/s41467-019-10842-5 |
Structure of the activated Edc1-Dcp1-Dcp2-Edc3 mRNA decapping complex with substrate analog poised for catalysis | |
Mugridge, Jeffrey S.1; Tibble, Ryan W.1,2; Ziemniak, Marcin3,4,5; Jemielity, Jacek4; Gross, John D.1,2 | |
2019-07-22 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Poland |
英文摘要 | The conserved decapping enzyme Dcp2 recognizes and removes the 5' eukaryotic cap from mRNA transcripts in a critical step of many cellular RNA decay pathways. Dcp2 is a dynamic enzyme that functions in concert with the essential activator Dcp1 and a diverse set of coactivators to selectively and efficiently decap target mRNAs in the cell. Here we present a 2.84 angstrom crystal structure of K. lactis Dcp1-Dcp2 in complex with coactivators Edc1 and Edc3, and with substrate analog bound to the Dcp2 active site. Our structure shows how Dcp2 recognizes cap substrate in the catalytically active conformation of the enzyme, and how coactivator Edc1 forms a three-way interface that bridges the domains of Dcp2 to consolidate the active conformation. Kinetic data reveal Dcp2 has selectivity for the first transcribed nucleotide during the catalytic step. The heterotetrameric Edc1-Dcp1-Dcp2-Edc3 structure shows how coactivators Edc1 and Edc3 can act simultaneously to activate decapping catalysis. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000427907900012 |
WOS关键词 | GENOME-WIDE ANALYSIS ; PROTEIN COMPLEXES ; CAP RECOGNITION ; ENZYME DCP2 ; DEGRADATION ; IDENTIFICATION ; SURVEILLANCE ; MECHANISMS ; DYNAMICS ; POMBE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204461 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94158 USA; 2.Univ Calif San Francisco, Program Chem & Chem Biol, San Francisco, CA 94158 USA; 3.Univ Warsaw, Fac Phys, Inst Expt Phys, Div Biophys, PL-02089 Warsaw, Poland; 4.Univ Warsaw, Ctr New Technol, PL-02097 Warsaw, Poland; 5.Univ Warsaw, Dept Chem, Biol & Chem Res Ctr, PL-02089 Warsaw, Poland |
推荐引用方式 GB/T 7714 | Mugridge, Jeffrey S.,Tibble, Ryan W.,Ziemniak, Marcin,et al. Structure of the activated Edc1-Dcp1-Dcp2-Edc3 mRNA decapping complex with substrate analog poised for catalysis[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Mugridge, Jeffrey S.,Tibble, Ryan W.,Ziemniak, Marcin,Jemielity, Jacek,&Gross, John D..(2019).Structure of the activated Edc1-Dcp1-Dcp2-Edc3 mRNA decapping complex with substrate analog poised for catalysis.NATURE COMMUNICATIONS,9. |
MLA | Mugridge, Jeffrey S.,et al."Structure of the activated Edc1-Dcp1-Dcp2-Edc3 mRNA decapping complex with substrate analog poised for catalysis".NATURE COMMUNICATIONS 9(2019). |
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