Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-019-10872-z |
Heterozygous diploid and interspecies SCRaMbLEing | |
Shen, Michael J.1,2; Wu, Yi3,4; Yang, Kun5,6; Li, Yunxiang3,4; Xu, Hui3,4; Zhang, Haoran3,4; Li, Bing-Zhi3,4; Li, Xia3,4; Xiao, Wen-Hai3,4; Zhou, Xiao3,4; Mitchell, Leslie A.1,2; Bader, Joel S.5,6; Yuan, Yingjin3,4; Boeke, Jef D.1,2 | |
2019-07-09 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Peoples R China |
英文摘要 | SCRaMbLE (Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution) is a genome restructuring technique that can be used in synthetic genomes such as that of Sc2.0, the synthetic yeast genome, which contains hundreds to thousands of strategically positioned loxPsym sites. SCRaMbLE has been used to induce rearrangements in yeast strains harboring one or more synthetic chromosomes, as well as plasmid DNA in vitro and in vivo. Here we describe a collection of heterozygous diploid strains produced by mating haploid semisynthetic Sc2.0 strains to haploid native parental strains. We subsequently demonstrate that such heterozygous diploid strains are more robust to the effects of SCRaMbLE than haploid semisynthetic strains, rapidly improve rationally selected phenotypes in SCRaMbLEd heterozygous diploids, and establish that multiple sets of independent genomic rearrangements are able to lead to similar phenotype enhancements. Finally, we show that heterozygous diploid SCRaMbLE can also be carried out in interspecies hybrid strains. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000432664900005 |
WOS关键词 | LIFE-SPAN ; YEAST ; DIVERSITY |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204445 |
专题 | 资源环境科学 |
作者单位 | 1.NYU Langone Hlth, Dept Biochem Mol Pharmacol, New York, NY 10016 USA; 2.NYU Langone Hlth, Inst Syst Genet, New York, NY 10016 USA; 3.Tianjin Univ, Sch Chem Engn & Technol, Minist Educ, Key Lab Syst Bioengn, Tianjin 300072, Peoples R China; 4.Tianjin Univ, Collaborat Innovat Ctr Chem Sci & Engn Tianjin, SynBio Res Platform, Tianjin 300072, Peoples R China; 5.Johns Hopkins Univ, Dept Biomed Engn, Baltimore, MD 21218 USA; 6.Johns Hopkins Univ, Sch Med, High Throughput Biol Ctr, Baltimore, MD 21205 USA |
推荐引用方式 GB/T 7714 | Shen, Michael J.,Wu, Yi,Yang, Kun,et al. Heterozygous diploid and interspecies SCRaMbLEing[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Shen, Michael J..,Wu, Yi.,Yang, Kun.,Li, Yunxiang.,Xu, Hui.,...&Boeke, Jef D..(2019).Heterozygous diploid and interspecies SCRaMbLEing.NATURE COMMUNICATIONS,9. |
MLA | Shen, Michael J.,et al."Heterozygous diploid and interspecies SCRaMbLEing".NATURE COMMUNICATIONS 9(2019). |
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