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DOI | 10.1038/s41467-019-10866-x |
Small GTPases and BAR domain proteins regulate branched actin polymerisation for clathrin and dynamin-independent endocytosis | |
Sathe, Mugdha1; Muthukrishnan, Gayatri1; Rae, James2,3; Disanza, Andrea4,5; Thattai, Mukund1,6; Scita, Giorgio4,5; Parton, Robert G.2,3; Mayor, Satyajit1,7 | |
2019-06-25 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | India; Australia; Italy |
英文摘要 | Using real-time TIRF microscopy imaging, we identify sites of clathrin and dynamin-independent CLIC/GEEC (CG) endocytic vesicle formation. This allows spatio-temporal localisation of known molecules affecting CG endocytosis; GBF1 (a GEF for ARF1), ARF1 and CDC42 which appear sequentially over 60 s, preceding scission. In an RNAi screen for BAR domain proteins affecting CG endocytosis, IRSp53 and PICK1, known interactors of CDC42 and ARF1, respectively, were selected. Removal of IRSp53, a negative curvature sensing protein, abolishes CG endocytosis. Furthermore, the identification of ARP2/3 complex at CG endocytic sites, maintained in an inactive state reveals a function for PICK1, an ARP2/3 inhibitor. The spatio-temporal sequence of the arrival and disappearance of the molecules suggest a mechanism for a clathrin and dynamin-independent endocytic process. Coincident with the loss of PICK1 by GBF1-activated ARF1, CDC42 recruitment leads to the activation of IRSp53 and the ARP2/3 complex, resulting in a burst of F-actin polymerisation potentially powering scission. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000431742300001 |
WOS关键词 | SMALL-MOLECULE INHIBITOR ; MEMBRANE SCISSION ; N-WASP ; IRSP53 ; CDC42 ; COMPLEX ; PICK1 ; IDENTIFICATION ; RECRUITMENT ; FILOPODIA |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204425 |
专题 | 资源环境科学 |
作者单位 | 1.Natl Ctr Biol Sci TIFR, Bellary Rd, Bangalore 560065, Karnataka, India; 2.Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia; 3.Univ Queensland, Ctr Microscopy & Microanal, Brisbane, Qld 4072, Australia; 4.Fdn Ist FIRC Oncol Mol, IFOM, I-20139 Milan, Italy; 5.Univ Milan, Dept Oncol & Hemato Oncol, I-20122 Milan, Italy; 6.Natl Ctr Biol Sci TIFR, Simons Ctr Study Living Machines, Bellary Rd, Bangalore 560065, Karnataka, India; 7.Inst Stem Cell Biol & Regenerat Med, Bellary Rd, Bangalore 560065, Karnataka, India |
推荐引用方式 GB/T 7714 | Sathe, Mugdha,Muthukrishnan, Gayatri,Rae, James,et al. Small GTPases and BAR domain proteins regulate branched actin polymerisation for clathrin and dynamin-independent endocytosis[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Sathe, Mugdha.,Muthukrishnan, Gayatri.,Rae, James.,Disanza, Andrea.,Thattai, Mukund.,...&Mayor, Satyajit.(2019).Small GTPases and BAR domain proteins regulate branched actin polymerisation for clathrin and dynamin-independent endocytosis.NATURE COMMUNICATIONS,9. |
MLA | Sathe, Mugdha,et al."Small GTPases and BAR domain proteins regulate branched actin polymerisation for clathrin and dynamin-independent endocytosis".NATURE COMMUNICATIONS 9(2019). |
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