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DOI | 10.1038/s41467-019-09971-8 |
The structural basis of proton driven zinc transport by ZntB | |
Gati, Cornelius1; Stetsenko, Artem2; Slotboom, Dirk J.2; Scheres, Sjors H. W.1; Guskov, Albert2 | |
2019-05-16 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | England; Netherlands |
英文摘要 | Zinc is an essential microelement to sustain all forms of life. However, excess of zinc is toxic, therefore dedicated import, export and storage proteins for tight regulation of the zinc concentration have evolved. In Enterobacteriaceae, several membrane transporters are involved in zinc homeostasis and linked to virulence. ZntB has been proposed to play a role in the export of zinc, but the transport mechanism of ZntB is poorly understood and based only on experimental characterization of its distant homologue CorA magnesium channel. Here, we report the cryo-electron microscopy structure of full-length ZntB from Escherichia coil together with the results of isothermal titration calorimetry, and radio-ligand uptake and fluorescent transport assays on ZntB reconstituted into liposomes. Our results show that ZntB mediates Zn2+ uptake, stimulated by a pH gradient across the membrane, using a transport mechanism that does not resemble the one proposed for homologous CorA channels. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000414376900014 |
WOS关键词 | CRYO-EM STRUCTURE ; MAGNESIUM ; MG2+ ; ION ; RESISTANCE ; HYDRATION ; CHANNEL ; SELECTIVITY ; MECHANISMS ; VIRULENCE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204370 |
专题 | 资源环境科学 |
作者单位 | 1.MRC, Lab Mol Biol, Francis Crick Ave,Cambridge Biomed Campus, Cambridge CB2 0QH, England; 2.Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, Nijenborgh 4, NL-9747 AG Groningen, Netherlands |
推荐引用方式 GB/T 7714 | Gati, Cornelius,Stetsenko, Artem,Slotboom, Dirk J.,et al. The structural basis of proton driven zinc transport by ZntB[J]. NATURE COMMUNICATIONS,2019,8. |
APA | Gati, Cornelius,Stetsenko, Artem,Slotboom, Dirk J.,Scheres, Sjors H. W.,&Guskov, Albert.(2019).The structural basis of proton driven zinc transport by ZntB.NATURE COMMUNICATIONS,8. |
MLA | Gati, Cornelius,et al."The structural basis of proton driven zinc transport by ZntB".NATURE COMMUNICATIONS 8(2019). |
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