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Most RNA folding studies have been performed under non-physiological conditions of high concentrations (>= 10 mM) of Mg-f(ree)2+, while actual cellular concentrations of Mg-free(2+) are only similar to 1 mM in a background of greater than 50 mM Mg-total(2+) . To uncover cellular behavior of RNA, we devised cytoplasm mimic systems that include biological concentrations of amino acids, which weakly chelate Mg2+. Amino acid-chelated Mg2+ (aaCM) of similar to 15 mM dramatically increases RNA folding and prevents RNA degradation. Furthermore, aaCM enhance self-cleavage of several different ribozymes, up to 100,000-fold at Mg-free(2+) of just 0.5 mM, indirectly through RNA compaction. Other metabolites that weakly chelate magnesium offer similar beneficial effects, which implies chelated magnesium may enhance RNA function in the cell in the same way. Overall, these results indicate that the states of Mg(2+ )should not be limited to free and bound only, as weakly bound Mg2+ strongly promotes RNA function under cellular conditions.