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DOI | 10.1038/s41467-019-08587-2 |
Cellular conditions of weakly chelated magnesium ions strongly promote RNA stability and catalysis | |
Yamagami, Ryota1,2; Bingaman, Jamie L.1,2,4; Frankel, Erica A.1,2,5; Bevilacqua, Philip C.1,2,3 | |
2019-02-15 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Most RNA folding studies have been performed under non-physiological conditions of high concentrations (>= 10 mM) of Mg-f(ree)2+, while actual cellular concentrations of Mg-free(2+) are only similar to 1 mM in a background of greater than 50 mM Mg-total(2+) . To uncover cellular behavior of RNA, we devised cytoplasm mimic systems that include biological concentrations of amino acids, which weakly chelate Mg2+. Amino acid-chelated Mg2+ (aaCM) of similar to 15 mM dramatically increases RNA folding and prevents RNA degradation. Furthermore, aaCM enhance self-cleavage of several different ribozymes, up to 100,000-fold at Mg-free(2+) of just 0.5 mM, indirectly through RNA compaction. Other metabolites that weakly chelate magnesium offer similar beneficial effects, which implies chelated magnesium may enhance RNA function in the cell in the same way. Overall, these results indicate that the states of Mg(2+ )should not be limited to free and bound only, as weakly bound Mg2+ strongly promotes RNA function under cellular conditions. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000433889400007 |
WOS关键词 | HAMMERHEAD RIBOZYME ; CRYSTAL-STRUCTURE ; MESSENGER-RNA ; ACID ; SECONDARY ; BINDING |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204225 |
专题 | 资源环境科学 |
作者单位 | 1.Penn State Univ, Dept Chem, University Pk, PA 16802 USA; 2.Penn State Univ, Ctr RNA Mol Biol, University Pk, PA 16802 USA; 3.Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA; 4.Immunome Inc, 665 Stockton Dr,Suite 300, Exton, PA 19341 USA; 5.Dow Chem Co USA, 400 Arcola Rd, Collegeville, PA 19426 USA |
推荐引用方式 GB/T 7714 | Yamagami, Ryota,Bingaman, Jamie L.,Frankel, Erica A.,et al. Cellular conditions of weakly chelated magnesium ions strongly promote RNA stability and catalysis[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Yamagami, Ryota,Bingaman, Jamie L.,Frankel, Erica A.,&Bevilacqua, Philip C..(2019).Cellular conditions of weakly chelated magnesium ions strongly promote RNA stability and catalysis.NATURE COMMUNICATIONS,9. |
MLA | Yamagami, Ryota,et al."Cellular conditions of weakly chelated magnesium ions strongly promote RNA stability and catalysis".NATURE COMMUNICATIONS 9(2019). |
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