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DOI | 10.1038/s41467-018-07164-3 |
Non-catalytic signaling by pseudokinase ILK for regulating cell adhesion | |
Vaynberg, Julia1; Fukuda, Koichi1; Lu, Fan1,2; Bialkowska, Katarzyna1; Chen, Yinghua3; Plow, Edward F.1; Qin, Jun1,2 | |
2018-10-26 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Dynamic communication between integrin-containing complexes (focal adhesions, FAs) and actin filaments is critical for regulating cell adhesion. Pseudokinase ILK plays a key role in this process but the underlying mechanism remains highly elusive. Here we show that by recruiting FA adaptors PINCH and Parvin into a heterotrimeric complex (IPP), ILK triggers Factin filament bundling - a process known to generate force/mechanical signal to promote cytoskeleton reassembly and dynamic cell adhesion. Structural, biochemical, and functional analyses revealed that the F-actin bundling is orchestrated by two previously unrecognized WASP-Homology-2 actin binding motifs within IPP, one from PINCH and the other from Parvin. Strikingly, this process is also sensitized to Mg-ATP bound to the pseudoactive site of ILK and its dysregulation severely impairs stress fibers formation, cell spreading, and migration. These data identify a crucial mechanism for ILK, highlighting its uniqueness as a pseudokinase to transduce non-catalytic signal and regulate cell adhesion. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000448414100015 |
WOS关键词 | INTEGRIN-LINKED KINASE ; COMPLEX REVEALS ; PROTEIN COMPLEXES ; FOCAL ADHESIONS ; MATRIX ADHESION ; ACTIN DYNAMICS ; ALPHA-PARVIN ; PINCH ; ADAPTER ; NMR |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204069 |
专题 | 资源环境科学 |
作者单位 | 1.Cleveland Clin, Lerner Res Inst, Dept Mol Cardiol, 9500 Euclid Ave, Cleveland, OH 44195 USA; 2.Case Western Reserve Univ, Sch Med, Dept Biochem, Cleveland, OH 44106 USA; 3.Case Western Reserve Univ, Sch Med, Dept Physiol & Biophys, Cleveland, OH 44106 USA |
推荐引用方式 GB/T 7714 | Vaynberg, Julia,Fukuda, Koichi,Lu, Fan,et al. Non-catalytic signaling by pseudokinase ILK for regulating cell adhesion[J]. NATURE COMMUNICATIONS,2018,9. |
APA | Vaynberg, Julia.,Fukuda, Koichi.,Lu, Fan.,Bialkowska, Katarzyna.,Chen, Yinghua.,...&Qin, Jun.(2018).Non-catalytic signaling by pseudokinase ILK for regulating cell adhesion.NATURE COMMUNICATIONS,9. |
MLA | Vaynberg, Julia,et al."Non-catalytic signaling by pseudokinase ILK for regulating cell adhesion".NATURE COMMUNICATIONS 9(2018). |
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