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DOI10.1038/s41467-018-06252-8
Picosecond orientational dynamics of water in living cells
Tros, Martijn1; Zheng, Linli2; Hunger, Johannes3; Bonn, Mischa3; Bonn, Daniel4; Smits, Gertien J.2; Woutersen, Sander1
2017-10-12
发表期刊NATURE COMMUNICATIONS
ISSN2041-1723
出版年2017
卷号8
文章类型Article
语种英语
国家Netherlands; Germany
英文摘要

Cells are extremely crowded, and a central question in biology is how this affects the intracellular water. Here, we use ultrafast vibrational spectroscopy and dielectric-relaxation spectroscopy to observe the random orientational motion of water molecules inside living cells of three prototypical organisms: Escherichia coli, Saccharomyces cerevisiae (yeast), and spores of Bacillus subtilis. In all three organisms, most of the intracellular water exhibits the same random orientational motion as neat water (characteristic time constants similar to 9 and similar to 2 ps for the first-order and second-order orientational correlation functions), whereas a smaller fraction exhibits slower orientational dynamics. The fraction of slow intracellular water varies between organisms, ranging from similar to 20% in E. coli to similar to 45% in B. subtilis spores. Comparison with the water dynamics observed in solutions mimicking the chemical composition of (parts of) the cytosol shows that the slow water is bound mostly to proteins, and to a lesser extent to other biomolecules and ions.


领域资源环境
收录类别SCI-E
WOS记录号WOS:000412860100004
WOS关键词2-DIMENSIONAL INFRARED-SPECTROSCOPY ; BACILLUS-SUBTILIS SPORES ; HYDROGEN-BOND NETWORK ; BACTERIAL-SPORES ; TERAHERTZ SPECTROSCOPY ; DIELECTRIC-RELAXATION ; HYDRATION DYNAMICS ; CORE WATER ; PROTEIN ; CYTOPLASM
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
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文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/204016
专题资源环境科学
作者单位1.Univ Amsterdam, Van t Hoff Inst Mol Sci, Sci Pk 904, NL-1098XH Amsterdam, Netherlands;
2.Univ Amsterdam, Swammerdam Inst Life Sci, Sci Pk 904, NL-1098XH Amsterdam, Netherlands;
3.Max Planck Inst Polymer Res, Dept Mol Spect, Ackermannweg 10, D-55128 Mainz, Germany;
4.Univ Amsterdam, Inst Phys, Sci Pk 904, NL-1098XH Amsterdam, Netherlands
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GB/T 7714
Tros, Martijn,Zheng, Linli,Hunger, Johannes,et al. Picosecond orientational dynamics of water in living cells[J]. NATURE COMMUNICATIONS,2017,8.
APA Tros, Martijn.,Zheng, Linli.,Hunger, Johannes.,Bonn, Mischa.,Bonn, Daniel.,...&Woutersen, Sander.(2017).Picosecond orientational dynamics of water in living cells.NATURE COMMUNICATIONS,8.
MLA Tros, Martijn,et al."Picosecond orientational dynamics of water in living cells".NATURE COMMUNICATIONS 8(2017).
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