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DOI | 10.1038/s41467-018-05280-8 |
An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila | |
Qiao, Huan-Huan1,2; Wang, Fang1; Xu, Rong-Gang1; Sun, Jin1,2; Zhu, Ruibao1,2; Mao, Decai1,3; Ren, Xingjie4; Wang, Xia1; Jia, Yu1,2,8; Peng, Ping1,2,8; Shen, Da1; Liu, Lu-Ping1,5; Chang, Zhijie6,7; Wang, Guirong8; Li, Shao9; Ji, Jun-Yuan10; Liu, Qingfei11; Ni, Jian-Quan1,12 | |
2018-10-08 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Peoples R China; USA |
英文摘要 | Being relatively simple and practical, Drosophila transgenic RNAi is the technique of top priority choice to quickly study genes with pleiotropic functions. However, drawbacks have emerged over time, such as high level of false positive and negative results. To overcome these shortcomings and increase efficiency, specificity and versatility, we develop a next generation transgenic RNAi system. With this system, the leaky expression of the basal promoter is significantly reduced, as well as the heterozygous ratio of transgenic RNAi flies. In addition, it has been first achieved to precisely and efficiently modulate highly expressed genes. Furthermore, we increase versatility which can simultaneously knock down multiple genes in one step. A case illustration is provided of how this system can be used to study the synthetic developmental effect of histone acetyltransferases. Finally, we have generated a collection of transgenic RNAi lines for those genes that are highly homologous to human disease genes. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000446567300002 |
WOS关键词 | TRANSCRIPTION FACTOR ; SCREEN ; GENES ; INTERFERENCE ; EXPRESSION ; IDENTIFICATION ; NEUROGENETICS ; MORPHOGENESIS ; MELANOGASTER ; REPRESSION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203903 |
专题 | 资源环境科学 |
作者单位 | 1.Tsinghua Univ, Sch Med, Gene Regulatory Lab, Beijing 100084, Peoples R China; 2.Tsinghua Univ Peking Univ Joint Ctr Life Sci, Beijing 100084, Peoples R China; 3.Sichuan Acad Grassland Sci, Chengdu, Sichuan, Peoples R China; 4.Univ Calif San Francisco, Dept Neurol, Inst Human Genet, San Francisco, CA 94143 USA; 5.Tsinghua Univ, Tsinghua Fly Ctr, Beijing 100084, Peoples R China; 6.Tsinghua Univ, State Key Lab Membrane Biol, Sch Med, Beijing 100084, Peoples R China; 7.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China; 8.Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China; 9.Tsinghua Univ, MTCM Ctr 10, MOE Key Lab Bioinformat, Bioinformat Div,Dept Automat,BNRIST, Beijing 100084, Peoples R China; 10.Texas A&M Hlth Sci Ctr, Dept Mol & Cellular Med, Coll Med, College Stn, TX 77843 USA; 11.Tsinghua Univ, Sch Pharmaceut Sci, Beijing 100084, Peoples R China; 12.Tongji Univ, Tsingdao Adv Res Inst, Qingdao 266000, Peoples R China |
推荐引用方式 GB/T 7714 | Qiao, Huan-Huan,Wang, Fang,Xu, Rong-Gang,et al. An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila[J]. NATURE COMMUNICATIONS,2018,9. |
APA | Qiao, Huan-Huan.,Wang, Fang.,Xu, Rong-Gang.,Sun, Jin.,Zhu, Ruibao.,...&Ni, Jian-Quan.(2018).An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila.NATURE COMMUNICATIONS,9. |
MLA | Qiao, Huan-Huan,et al."An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila".NATURE COMMUNICATIONS 9(2018). |
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