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DOI | 10.1038/s41467-018-04971-6 |
Calcium stabilizes the strongest protein fold | |
Milles, Lukas F.1,2; Unterauer, Eduard M.1,2; Nicolaus, Thomas1,2; Gaub, Hermann E.1,2 | |
2018-11-12 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany |
英文摘要 | Staphylococcal pathogens adhere to their human targets with exceptional resilience to mechanical stress, some propagating force to the bacterium via small, Ig-like folds called B domains. We examine the mechanical stability of these folds using atomic force microscopybased single-molecule force spectroscopy. The force required to unfold a single B domain is larger than 2 nN - the highest mechanostability of a protein to date by a large margin. B domains coordinate three calcium ions, which we identify as crucial for their extreme mechanical strength. When calcium is removed through chelation, unfolding forces drop by a factor of four. Through systematic mutations in the calcium coordination sites we can tune the unfolding forces from over 2 nN to 0.15 nN, and dissect the contribution of each ion to B domain mechanostability. Their extraordinary strength, rapid refolding and calcium-tunable force response make B domains interesting protein design targets. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000449763100020 |
WOS关键词 | MOLECULE FORCE SPECTROSCOPY ; AUREUS CLUMPING FACTOR ; STRUCTURAL MODEL ; BINDING ; DYNAMICS ; SURFACE ; MECHANISM ; ADHESION ; PILI ; SDRD |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203859 |
专题 | 资源环境科学 |
作者单位 | 1.Ludwig Maximilians Univ Munchen, Angew Phys, Amalienstr 54, D-80799 Munich, Germany; 2.Ludwig Maximilians Univ Munchen, Ctr Nanosci, Amalienstr 54, D-80799 Munich, Germany |
推荐引用方式 GB/T 7714 | Milles, Lukas F.,Unterauer, Eduard M.,Nicolaus, Thomas,et al. Calcium stabilizes the strongest protein fold[J]. NATURE COMMUNICATIONS,2018,9. |
APA | Milles, Lukas F.,Unterauer, Eduard M.,Nicolaus, Thomas,&Gaub, Hermann E..(2018).Calcium stabilizes the strongest protein fold.NATURE COMMUNICATIONS,9. |
MLA | Milles, Lukas F.,et al."Calcium stabilizes the strongest protein fold".NATURE COMMUNICATIONS 9(2018). |
条目包含的文件 | 条目无相关文件。 |
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