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DOI | 10.1038/s41467-018-03506-3 |
Understanding CRY2 interactions for optical control of intracellular signaling | |
Duan, Liting1; Hope, Jen1; Ong, Qunxiang1; Lou, Hsin-Ya1; Kim, Namdoo2,3,4; McCarthy, Comfrey5; Acero, Victor6; Lin, Michael Z.2,3,4; Cui, Bianxiao1 | |
2017-09-15 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Arabidopsis cryptochrome 2 (CRY2) can simultaneously undergo light-dependent CRY2-CRY2 homo-oligomerization and CRY2-CIB1 hetero-dimerization, both of which have been widely used to optically control intracellular processes. Applications using CRY2-CIB1 interaction desire minimal CRY2 homo-oligomerization to avoid unintended complications, while those utilizing CRY2-CRY2 interaction prefer robust homo-oligomerization. However, selecting the type of CRY2 interaction has not been possible as the molecular mechanisms underlying CRY2 interactions are unknown. Here we report CRY2-CIB1 and CRY2-CRY2 interactions are governed by well-separated protein interfaces at the two termini of CRY2. N-terminal charges are critical for CRY2-CIB1 interaction. Moreover, two C-terminal charges impact CRY2 homo-oligomerization, with positive charges facilitating oligomerization and negative charges inhibiting it. By engineering C-terminal charges, we develop CRY2high and CRY2low with elevated or suppressed oligomerization respectively, which we use to tune the levels of Raf/MEK/ERK signaling. These results contribute to our understanding of the mechanisms underlying light-induced CRY2 interactions and enhance the controllability of CRY2-based optogenetic systems. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000410811900004 |
WOS关键词 | BLUE-LIGHT ; OPTOGENETIC CONTROL ; MAMMALIAN-CELLS ; SPATIOTEMPORAL CONTROL ; PROTEIN INTERACTIONS ; CRYPTOCHROME 2 ; LIVING CELLS ; ARABIDOPSIS ; DOMAIN ; ACTIVATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203738 |
专题 | 资源环境科学 |
作者单位 | 1.Stanford Univ, Dept Chem, Stanford, CA 94305 USA; 2.Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA; 3.Stanford Univ, Dept Pediat, Stanford, CA 94305 USA; 4.Stanford Univ, Dept Neurobiol, Stanford, CA 94305 USA; 5.Northeastern Univ, Dept Psychol, Boston, MA 02115 USA; 6.Penn State Univ, Dept Engn Sci, University Pk, PA 16802 USA |
推荐引用方式 GB/T 7714 | Duan, Liting,Hope, Jen,Ong, Qunxiang,et al. Understanding CRY2 interactions for optical control of intracellular signaling[J]. NATURE COMMUNICATIONS,2017,8. |
APA | Duan, Liting.,Hope, Jen.,Ong, Qunxiang.,Lou, Hsin-Ya.,Kim, Namdoo.,...&Cui, Bianxiao.(2017).Understanding CRY2 interactions for optical control of intracellular signaling.NATURE COMMUNICATIONS,8. |
MLA | Duan, Liting,et al."Understanding CRY2 interactions for optical control of intracellular signaling".NATURE COMMUNICATIONS 8(2017). |
条目包含的文件 | 条目无相关文件。 |
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