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DOI | 10.1038/s41467-017-01456-w |
Visualizing conformation transitions of the Lipid II flippase MurJ | |
Kuk, Alvin C. Y.; Hao, Aili; Guan, Ziqiang; Lee, Seok-Yong | |
2019-04-15 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The biosynthesis of many polysaccharides, including bacterial peptidoglycan and eukaryotic N-linked glycans, requires transport of lipid-linked oligosaccharide (LLO) precursors across the membrane by specialized flippases. MurJ is the flippase for the lipid-linked peptidoglycan precursor Lipid II, a key player in bacterial cell wall synthesis, and a target of recently discovered antibacterials. However, the flipping mechanism of LLOs including Lipid II remains poorly understood due to a dearth of structural information. Here we report crystal structures of MurJ captured in inward-closed, inward-open, inward-occluded and outward-facing conformations. Together with mutagenesis studies, we elucidate the conformational transitions in MurJ that mediate lipid flipping, identify the key ion for function, and provide a framework for the development of inhibitors. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000464494100005 |
WOS关键词 | CRYSTAL-STRUCTURE ; MOLECULAR-DYNAMICS ; ANTIBIOTICS ; INHIBITION ; TRANSLOCATION ; REVEALS ; PDB2PQR ; BINDING ; MVIN |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203487 |
专题 | 资源环境科学 |
作者单位 | Duke Univ, Dept Biochem, Med Ctr, 303 Res Dr, Durham, NC 27710 USA |
推荐引用方式 GB/T 7714 | Kuk, Alvin C. Y.,Hao, Aili,Guan, Ziqiang,et al. Visualizing conformation transitions of the Lipid II flippase MurJ[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Kuk, Alvin C. Y.,Hao, Aili,Guan, Ziqiang,&Lee, Seok-Yong.(2019).Visualizing conformation transitions of the Lipid II flippase MurJ.NATURE COMMUNICATIONS,10. |
MLA | Kuk, Alvin C. Y.,et al."Visualizing conformation transitions of the Lipid II flippase MurJ".NATURE COMMUNICATIONS 10(2019). |
条目包含的文件 | 条目无相关文件。 |
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