Automating multimodal microscopy with NanoJ-Fluidics

doi:10.1038/s41467-017-01228-6

GSTDTAP > 资源环境科学

DOI	10.1038/s41467-017-01228-6
	Automating multimodal microscopy with NanoJ-Fluidics
	Almada, Pedro 1,2; Pereira, Pedro M.1,2,3; Cuiley, Sian 1,2,3; Caillol, Ghislaine 4; Boroni-Rueda, Fanny 4; Dix, Christina L.1; Charras, Guillaume 5,6; Baum, Buzz 1,6; Laine, Romain F.1,3; Leterrier, Christophe 4; Henriques, Ricardo 1,2,3
	2019-03-15
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
出版年	2019
卷号	10
文章类型	Article
语种	英语
国家	England; France
英文摘要	Combining and multiplexing microscopy approaches is crucial to understand cellular events, but requires elaborate workflows. Here, we present a robust, open-source approach for treating, labelling and imaging live or fixed cells in automated sequences. NanoJ-Fluidics is based on low-cost Lego hardware controlled by ImageJ-based software, making high-content, multimodal imaging easy to implement on any microscope with high reproducibility. We demonstrate its capacity on event-driven, super-resolved live-to-fixed and multiplexed STORM/DNA-PAINT experiments.
领域	资源环境
收录类别	SCI-E
WOS记录号	WOS:000461311100003
WOS关键词	SUPERRESOLUTION MICROSCOPY ; IMAGE ; ARCHITECTURE ; REVEALS ; BINDING ; PROBES ; PAINT
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
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引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/203481
专题	资源环境科学
作者单位	1.UCL, MRC Lab Mol Cell Biol, London WC1E 6BT, England; 2.UCL, Dept Cell & Dev Biol, London WC1E 6BT, England; 3.Francis Crick Inst, London NW1 1AT, England; 4.Aix Marseille Univ, CNRS, INP UMR7051, NeuroCyto, F-13015 Marseille, France; 5.London Ctr Nanotechnol, London WC1H 0AH, England; 6.UCL, Inst Phys Living Syst, London WC1E 6BT, England
推荐引用方式 GB/T 7714	Almada, Pedro,Pereira, Pedro M.,Cuiley, Sian,et al. Automating multimodal microscopy with NanoJ-Fluidics[J]. NATURE COMMUNICATIONS,2019,10.
APA	Almada, Pedro.,Pereira, Pedro M..,Cuiley, Sian.,Caillol, Ghislaine.,Boroni-Rueda, Fanny.,...&Henriques, Ricardo.(2019).Automating multimodal microscopy with NanoJ-Fluidics.NATURE COMMUNICATIONS,10.
MLA	Almada, Pedro,et al."Automating multimodal microscopy with NanoJ-Fluidics".NATURE COMMUNICATIONS 10(2019).