Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-017-01228-6 |
Automating multimodal microscopy with NanoJ-Fluidics | |
Almada, Pedro1,2; Pereira, Pedro M.1,2,3; Cuiley, Sian1,2,3; Caillol, Ghislaine4; Boroni-Rueda, Fanny4; Dix, Christina L.1; Charras, Guillaume5,6; Baum, Buzz1,6; Laine, Romain F.1,3; Leterrier, Christophe4; Henriques, Ricardo1,2,3 | |
2019-03-15 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | England; France |
英文摘要 | Combining and multiplexing microscopy approaches is crucial to understand cellular events, but requires elaborate workflows. Here, we present a robust, open-source approach for treating, labelling and imaging live or fixed cells in automated sequences. NanoJ-Fluidics is based on low-cost Lego hardware controlled by ImageJ-based software, making high-content, multimodal imaging easy to implement on any microscope with high reproducibility. We demonstrate its capacity on event-driven, super-resolved live-to-fixed and multiplexed STORM/DNA-PAINT experiments. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000461311100003 |
WOS关键词 | SUPERRESOLUTION MICROSCOPY ; IMAGE ; ARCHITECTURE ; REVEALS ; BINDING ; PROBES ; PAINT |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203481 |
专题 | 资源环境科学 |
作者单位 | 1.UCL, MRC Lab Mol Cell Biol, London WC1E 6BT, England; 2.UCL, Dept Cell & Dev Biol, London WC1E 6BT, England; 3.Francis Crick Inst, London NW1 1AT, England; 4.Aix Marseille Univ, CNRS, INP UMR7051, NeuroCyto, F-13015 Marseille, France; 5.London Ctr Nanotechnol, London WC1H 0AH, England; 6.UCL, Inst Phys Living Syst, London WC1E 6BT, England |
推荐引用方式 GB/T 7714 | Almada, Pedro,Pereira, Pedro M.,Cuiley, Sian,et al. Automating multimodal microscopy with NanoJ-Fluidics[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Almada, Pedro.,Pereira, Pedro M..,Cuiley, Sian.,Caillol, Ghislaine.,Boroni-Rueda, Fanny.,...&Henriques, Ricardo.(2019).Automating multimodal microscopy with NanoJ-Fluidics.NATURE COMMUNICATIONS,10. |
MLA | Almada, Pedro,et al."Automating multimodal microscopy with NanoJ-Fluidics".NATURE COMMUNICATIONS 10(2019). |
条目包含的文件 | 条目无相关文件。 |
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