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DOI | 10.1038/ncomms15991 |
The pause-initiation limit restricts transcription activation in human cells | |
Gressel, Saskia; Schwalb, Bjoern; Cramer, Patrick | |
2019-08-09 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany |
英文摘要 | Eukaryotic gene transcription is often controlled at the level of RNA polymerase II (Pol II) pausing in the promoter-proximal region. Pausing Pol II limits the frequency of transcription initiation ('pause-initiation limit'), predicting that the pause duration must be decreased for transcriptional activation. To test this prediction, we conduct a genome-wide kinetic analysis of the heat shock response in human cells. We show that the pause-initiation limit restricts transcriptional activation at most genes. Gene activation generally requires the activity of the P-TEFb kinase CDK9, which decreases the duration of Pol II pausing and thereby enables an increase in the productive initiation frequency. The transcription of enhancer elements is generally not pause limited and can be activated without CDK9 activity. Our results define the kinetics of Pol II transcriptional regulation in human cells at all gene classes during a natural transcription response. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000480234500017 |
WOS关键词 | RNA-POLYMERASE-II ; HEAT-SHOCK RESPONSE ; C-TERMINAL DOMAIN ; P-TEFB KINASE ; NASCENT RNA ; BINDING PROTEIN ; HSP70 GENE ; PROMOTER ; ELONGATION ; PHOSPHORYLATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203362 |
专题 | 资源环境科学 |
作者单位 | Max Planck Inst Biophys Chem, Dept Mol Biol, Fassberg 11, D-37077 Gottingen, Germany |
推荐引用方式 GB/T 7714 | Gressel, Saskia,Schwalb, Bjoern,Cramer, Patrick. The pause-initiation limit restricts transcription activation in human cells[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Gressel, Saskia,Schwalb, Bjoern,&Cramer, Patrick.(2019).The pause-initiation limit restricts transcription activation in human cells.NATURE COMMUNICATIONS,10. |
MLA | Gressel, Saskia,et al."The pause-initiation limit restricts transcription activation in human cells".NATURE COMMUNICATIONS 10(2019). |
条目包含的文件 | 条目无相关文件。 |
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