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DOI | 10.1038/ncomms14884 |
Long-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro | |
Fischer, Carola1; Milting, Hendrik2; Fein, Evelyn1; Reiser, Elisabeth1; Lu, Kun1; Seidel, Thomas3; Schinner, Camilla4; Schwarzmayr, Thomas5; Schramm, Rene2,6; Tomasi, Roland1,7; Husse, Britta1; Cao-Ehlker, Xiaochun1; Pohl, Ulrich1,8; Dendorfer, Andreas1,8 | |
2019-01-10 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany |
英文摘要 | In vitro models incorporating the complexity and function of adult human tissues are highly desired for translational research. Whilst vital slices of human myocardium approach these demands, their rapid degeneration in tissue culture precludes long-term experimentation. Here, we report preservation of structure and performance of human myocardium under conditions of physiological preload, compliance, and continuous excitation. In biomimetic culture, tissue slices prepared from explanted failing human hearts attain a stable state of contractility that can be monitored for up to 4 months or 2000000 beats in vitro. Cultured myocardium undergoes particular alterations in biomechanics, structure, and mRNA expression. The suitability of the model for drug safety evaluation is exemplified by repeated assessment of refractory period that permits sensitive analysis of repolarization impairment induced by the multimodal hERG-inhibitor pentamidine. Biomimetic tissue culture will provide new opportunities to study drug targets, gene functions, and cellular plasticity in adult human myocardium. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000455354800014 |
WOS关键词 | TRANSVERSE TUBULAR SYSTEM ; CARDIAC TISSUE ; MATURATION ; SLICES ; STRATEGIES ; CULTURE ; DRUG ; RAT |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203253 |
专题 | 资源环境科学 |
作者单位 | 1.Ludwig Maximilians Univ Munchen, Univ Hosp, Walter Brendel Ctr Expt Med, Marchioninistr 27, D-81377 Munich, Germany; 2.Ruhr Univ Bochum, Erich & Hanna Klessmann Inst, Heart & Diabet Ctr NRW, Clin Thorac & Cardiovasc Surg, Georgstr 11, D-32545 Bad Oeynhausen, Germany; 3.Friedrich Alexander Univ Erlangen Nurnberg, Inst Cellular & Mol Physiol, Waldstr 8, D-91054 Erlangen, Germany; 4.Ludwig Maximilians Univ Munchen, Inst Anat, Pettenkoferstr 11, D-80336 Munich, Germany; 5.Helmholtz Zentrum Munchen, Inst Human Genet, Ingolstadter Landstr 1, D-85764 Neuherberg, Germany; 6.Ludwig Maximilians Univ Munchen, Univ Hosp, Clin Cardiac Surg, Marchioninistr 15, D-81377 Munich, Germany; 7.Ludwig Maximilians Univ Munchen, Univ Hosp, Clin Anaesthesiol, Marchioninistr 15, D-81377 Munich, Germany; 8.Munich Heart Alliance, German Ctr Cardiovasc Res, Partner Site, D-80802 Munich, Germany |
推荐引用方式 GB/T 7714 | Fischer, Carola,Milting, Hendrik,Fein, Evelyn,et al. Long-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Fischer, Carola.,Milting, Hendrik.,Fein, Evelyn.,Reiser, Elisabeth.,Lu, Kun.,...&Dendorfer, Andreas.(2019).Long-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro.NATURE COMMUNICATIONS,10. |
MLA | Fischer, Carola,et al."Long-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro".NATURE COMMUNICATIONS 10(2019). |
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