Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/ncomms14393 |
Engineered ribosomes with tethered subunits for expanding biological function | |
Carlson, Erik D.1; 39;Aquino, Anne E.2 | |
2019-09-02 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Ribo-T is a ribosome with covalently tethered subunits where core 16S and 23S ribosomal RNAs form a single chimeric molecule. Ribo-T makes possible a functionally orthogonal ribosome-mRNA system in cells. Unfortunately, use of Ribo-T has been limited because of low activity of its original version. Here, to overcome this limitation, we use an evolutionary approach to select new tether designs that are capable of supporting faster cell growth and increased protein expression. Further, we evolve new orthogonal Ribo-T/mRNA pairs that function in parallel with, but independent of, natural ribosomes and mRNAs, increasing the efficiency of orthogonal protein expression. The Ribo-T with optimized designs is able to synthesize a diverse set of proteins, and can also incorporate multiple non-canonical amino acids into synthesized polypeptides. The enhanced Ribo-T designs should be useful for exploring poorly understood functions of the ribosome and engineering ribosomes with altered catalytic properties. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000483305400011 |
WOS关键词 | GENETIC-CODE ; AMINO-ACIDS ; TRANSLATION ; EVOLUTION ; MULTIPLE ; SYSTEM ; TRANSCRIPTION ; BIOGENESIS ; MECHANISM |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203225 |
专题 | 资源环境科学 |
作者单位 | 1.Northwestern Univ, Dept Chem & Biol Engn, 2145 Sheridan Rd,Tech E-136, Evanston, IL 60208 USA; 2.Northwestern Univ, Chem Life Proc Inst, 2170 Campus Dr, Evanston, IL 60208 USA; 3.Northwestern Univ, Ctr Synthet Biol, 2145 Sheridan Rd, Evanston, IL 60208 USA; 4.Northwestern Univ, Interdisciplinary Biol Sci Grad Program, Hogan 2-100,2205 Tech Dr, Evanston, IL 60208 USA; 5.Univ Illinois, Ctr Pharmaceut Biotechnol, Chicago, IL 60607 USA; 6.Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USA; 7.Johnson & Wales Univ, Dept Biol, Providence, RI 02903 USA |
推荐引用方式 GB/T 7714 | Carlson, Erik D.,39;Aquino, Anne E.. Engineered ribosomes with tethered subunits for expanding biological function[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Carlson, Erik D.,&39;Aquino, Anne E..(2019).Engineered ribosomes with tethered subunits for expanding biological function.NATURE COMMUNICATIONS,10. |
MLA | Carlson, Erik D.,et al."Engineered ribosomes with tethered subunits for expanding biological function".NATURE COMMUNICATIONS 10(2019). |
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