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DOI | 10.1038/ncomms14096 |
Regulation of in vivo dynein force production by CDK5 and 14-3-3 epsilon and KIAAO528 | |
Chapman, Dail E.1; Reddy, Babu J. N.1; Huy, Bunchhin1; Bovyn, Matthew J.1; Cruz, Stephen John S.1; Al-Shammari, Zahraa M.1; Han, Han1; Wang, Wenqi1; Smith, Deanna S.2; Gross, Steven P.1 | |
2019-01-16 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Single-molecule cytoplasmic dynein function is well understood, but there are major gaps in mechanistic understanding of cellular dynein regulation. We reported a mode of dynein regulation, force adaptation, where lipid droplets adapt to opposition to motion by increasing the duration and magnitude of force production, and found LIS1 and NudEL to be essential. Adaptation reflects increasing NudEL-LIS1 utilization; here, we hypothesize that such increasing utilization reflects CDK5-mediated NudEL phosphorylation, which increases the dynein-NudEL interaction, and makes force adaptation possible. We report that CDK5, 14-3-3 epsilon, and CDK5 cofactor KIAA0528 together promote NudEL phosphorylation and are essential for force adaptation. By studying the process in COS-1 cells lacking Tau, we avoid confounding neuronal effects of CDK5 on microtubules. Finally, we extend this in vivo regulatory pathway to lysosomes and mitochondria. Ultimately, we show that dynein force adaptation can control the severity of lysosomal tug-of-wars among other intracellular transport functions involving high force. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000455761000002 |
WOS关键词 | TUG-OF-WAR ; NEURONAL MIGRATION ; TRANSPORT ; LIS1 ; MECHANISM ; GLUT4 ; NUDE ; ACTIVATION ; INSULIN ; COMPLEX |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203163 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Calif Irvine, Dev & Cell Biol & Phys, Irvine, CA 92697 USA; 2.Univ South Carolina, Biol Sci, Columbia, SC USA |
推荐引用方式 GB/T 7714 | Chapman, Dail E.,Reddy, Babu J. N.,Huy, Bunchhin,et al. Regulation of in vivo dynein force production by CDK5 and 14-3-3 epsilon and KIAAO528[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Chapman, Dail E..,Reddy, Babu J. N..,Huy, Bunchhin.,Bovyn, Matthew J..,Cruz, Stephen John S..,...&Gross, Steven P..(2019).Regulation of in vivo dynein force production by CDK5 and 14-3-3 epsilon and KIAAO528.NATURE COMMUNICATIONS,10. |
MLA | Chapman, Dail E.,et al."Regulation of in vivo dynein force production by CDK5 and 14-3-3 epsilon and KIAAO528".NATURE COMMUNICATIONS 10(2019). |
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