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DOI | 10.1038/nature21412 |
Gamete fusion triggers bipartite transcription factor assembly to block re-fertilization | |
Vjestica, Aleksandar; Merlini, Laura; Nkosi, Pedro Junior; Martin, Sophie G. | |
2018-08-16 | |
发表期刊 | NATURE
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ISSN | 0028-0836 |
EISSN | 1476-4687 |
出版年 | 2018 |
卷号 | 560期号:7718页码:397-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Switzerland |
英文摘要 | The ploidy cycle, which is integral to sexual reproduction, requires meiosis to halve chromosome numbers as well as mechanisms that ensure zygotes are formed by exactly two partners(1-4). During sexual reproduction of the fungal model organism Schizosaccharomyces pombe, haploid P and M cells fuse to form a diploid zygote that immediately enters meiosis(5). Here we reveal that rapid post-fusion reconstitution of a bipartite transcription factor blocks re-fertilization. We first identify mutants that undergo transient cell fusion involving cytosol exchange but not karyogamy, and show that this drives distinct cell fates in the two gametes. The P partner undergoes lethal haploid meiosis, whereas the M cell persists in mating. The zygotic transcription that drives meiosis is rapidly initiated first from the P parental genome, even in wild-type cells. This asymmetric gene expression depends on a bipartite complex formed post-fusion between the cytosolic M-cell-specific peptide Mi and the nuclear P-cell-specific homeobox protein Pi(6,7), which captures Mi in the P nucleus. Zygotic transcription is thus poised to initiate in the P nucleus as fast as Mi reaches it after fusion, a design that we reconstruct using two synthetic interactors localized to the nucleus and the cytosol of two respective partner cells. Notably, delaying zygotic transcription-by postponing Mi expression or deleting its transcriptional target in the P genome-leads to zygotes fusing with additional gametes, thus forming polyploids and eventually aneuploid progeny. The signalling cascade to block re-fertilization shares components with, but bifurcates from, meiotic induction(8-10). Thus, a cytoplasmic connection upon gamete fusion leads to asymmetric reconstitution of a bipartite transcription factor to rapidly block re-fertilization and induce meiosis, ensuring genome maintenance during sexual reproduction. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000441673400046 |
WOS关键词 | FISSION YEAST ; SCHIZOSACCHAROMYCES-POMBE ; FORMIN FOR3P ; MEIOSIS-I ; EFFICIENT ; DYNAMICS ; ACTIVATION ; INHIBITOR ; EVOLUTION ; MODULES |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/202708 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | Univ Lausanne, Dept Fundamental Microbiol, Lausanne, Switzerland |
推荐引用方式 GB/T 7714 | Vjestica, Aleksandar,Merlini, Laura,Nkosi, Pedro Junior,et al. Gamete fusion triggers bipartite transcription factor assembly to block re-fertilization[J]. NATURE,2018,560(7718):397-+. |
APA | Vjestica, Aleksandar,Merlini, Laura,Nkosi, Pedro Junior,&Martin, Sophie G..(2018).Gamete fusion triggers bipartite transcription factor assembly to block re-fertilization.NATURE,560(7718),397-+. |
MLA | Vjestica, Aleksandar,et al."Gamete fusion triggers bipartite transcription factor assembly to block re-fertilization".NATURE 560.7718(2018):397-+. |
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