Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aaw9157 |
The nucleolus functions as a phase-separated protein quality control compartment | |
Frottin, F.1; Schueder, F.2,3,4; Tiwary, S.5; Gupta, R.1,7; Koerner, R.1; Schlichthaerle, T.2,3,4; Cox, J.5; Jungmann, R.2,3,4; Hartl, F. U.1,6; Hipp, M. S.1,6 | |
2019-07-26 | |
发表期刊 | SCIENCE
![]() |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 365期号:6451页码:342-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Germany; Denmark |
英文摘要 | The nuclear proteome is rich in stress-sensitive proteins, which suggests that effective protein quality control mechanisms are in place to ensure conformational maintenance. We investigated the role of the nucleolus in this process. In mammalian tissue culture cells under stress conditions, misfolded proteins entered the granular component (GC) phase of the nucleolus. Transient associations with nucleolar proteins such as NPM1 conferred low mobility to misfolded proteins within the liquid-like GC phase, avoiding irreversible aggregation. Refolding and extraction of proteins from the nucleolus during recovery from stress was Hsp70-dependent. The capacity of the nucleolus to store misfolded proteins was limited, and prolonged stress led to a transition of the nucleolar matrix from liquid-like to solid, with loss of reversibility and dysfunction in quality control. Thus, we suggest that the nucleolus has chaperone-like properties and can promote nuclear protein maintenance under stress. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000477703100033 |
WOS关键词 | DIPEPTIDE-REPEAT PROTEINS ; HEAT-SHOCK-PROTEIN ; NUCLEOCYTOPLASMIC TRANSPORT ; SUBCELLULAR-DISTRIBUTION ; ENDOPLASMIC-RETICULUM ; HEXANUCLEOTIDE REPEAT ; AGGREGATES SEQUESTER ; NUCLEAR ; C9ORF72 ; LOCALIZATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/201946 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Max Planck Inst Biochem, Dept Cellular Biochem, D-82152 Martinsried, Germany; 2.Max Planck Inst Biochem, Res Grp Mol Imaging & Bionanotechnol, D-82152 Martinsried, Germany; 3.Ludwig Maximilians Univ Munchen, Fac Phys, D-80539 Munich, Germany; 4.Ludwig Maximilians Univ Munchen, Ctr Nanosci, D-80539 Munich, Germany; 5.Max Planck Inst Biochem, Res Grp Computat Syst Biochem, D-82152 Martinsried, Germany; 6.Munich Cluster Syst Neurol SyNergy, D-80336 Munich, Germany; 7.Univ Copenhagen, Fac Hlth & Med Sci, Novo Nordisk Fdn Ctr Prot Res, DK-2200 Copenhagen, Denmark |
推荐引用方式 GB/T 7714 | Frottin, F.,Schueder, F.,Tiwary, S.,et al. The nucleolus functions as a phase-separated protein quality control compartment[J]. SCIENCE,2019,365(6451):342-+. |
APA | Frottin, F..,Schueder, F..,Tiwary, S..,Gupta, R..,Koerner, R..,...&Hipp, M. S..(2019).The nucleolus functions as a phase-separated protein quality control compartment.SCIENCE,365(6451),342-+. |
MLA | Frottin, F.,et al."The nucleolus functions as a phase-separated protein quality control compartment".SCIENCE 365.6451(2019):342-+. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论