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DOI | 10.1126/science.aaw4295 |
Protein crystallization promotes type 2 immunity and is reversible by antibody treatment | |
Persson, Emma K.1,2; Verstraete, Kenneth3,4; Heyndrickx, Ines1,2; Gevaert, Elien5; Aegerter, Helena1,2; Percier, Jean-Michel6; Deswarte, Kim1,2; Verschueren, Koen H. G.3,4; Dansercoer, Ann3,4; Gras, Delphine7; Chanez, Pascal7,8; Bachert, Claus5,9; Goncalves, Amanda10,11; Van Gorp, Hanne1,2; De Haard, Hans6; Blanchetot, Christophe6; Saunders, Michael6; Hammad, Hamida2; Savvides, Savvas N.3,4; Lambrecht, Bart N.1,2,12 | |
2019-05-24 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 364期号:6442页码:751-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Belgium; France; Sweden; Netherlands |
英文摘要 | Although spontaneous protein crystallization is a rare event in vivo, Charcot-Leyden crystals (CLCs) consisting of galectin-10 (Gal10) protein are frequently observed in eosinophilic diseases, such as asthma. We found that CLCs derived from patients showed crystal packing and Gal10 structure identical to those of Gal10 crystals grown in vitro. When administered to the airways, crystalline Gal10 stimulated innate and adaptive immunity and acted as a type 2 adjuvant. By contrast, a soluble Gal10 mutein was inert. Antibodies directed against key epitopes of the CLC crystallization interface dissolved preexisting CLCs in patient-derived mucus within hours and reversed crystal-driven inflammation, goblet-cell metaplasia, immunoglobulin E (IgE) synthesis, and bronchial hyperreactivity (BHR) in a humanized mouse model of asthma. Thus, protein crystals may promote hallmark features of asthma and are targetable by crystal-dissolving antibodies. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000469296000035 |
WOS关键词 | LEYDEN CRYSTAL PROTEIN ; MAJOR BASIC-PROTEIN ; EOSINOPHIL LYSOPHOSPHOLIPASE ; BIOCHEMICAL-CHARACTERIZATION ; URIC-ACID ; INFLAMMATION ; ASTHMA ; GALECTIN-10 ; MODEL ; ACTIVATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/201478 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.VIB Ctr Inflammat Res, Immunoregulat Unit, Ghent, Belgium; 2.Univ Ghent, Dept Internal Med & Pediat, Ghent, Belgium; 3.VIB Ctr Inflammat Res, Unit Struct Biol, Ghent, Belgium; 4.Univ Ghent, Dept Biochem & Microbiol, Ghent, Belgium; 5.Ghent Univ Hosp, ENT Dept, Upper Airways Res Lab, Ghent, Belgium; 6.arGEN X, Ghent, Belgium; 7.Aix Marseille Univ, INSERM, INRA, C2VN, Marseille, France; 8.Hop Nord Marseille, AP HM, Clin Bronches Allergies & Sommeil, Marseille, France; 9.Karolinska Inst, Div ENT Dis, CLINTEC, Stockholm, Sweden; 10.VIB Inflammat Res Ctr, BioImaging Core, Ghent, Belgium; 11.Univ Ghent, Dept Biomed Mol Biol, Ghent, Belgium; 12.ErasmusMC, Dept Pulm Med, Rotterdam, Netherlands |
推荐引用方式 GB/T 7714 | Persson, Emma K.,Verstraete, Kenneth,Heyndrickx, Ines,et al. Protein crystallization promotes type 2 immunity and is reversible by antibody treatment[J]. SCIENCE,2019,364(6442):751-+. |
APA | Persson, Emma K..,Verstraete, Kenneth.,Heyndrickx, Ines.,Gevaert, Elien.,Aegerter, Helena.,...&Lambrecht, Bart N..(2019).Protein crystallization promotes type 2 immunity and is reversible by antibody treatment.SCIENCE,364(6442),751-+. |
MLA | Persson, Emma K.,et al."Protein crystallization promotes type 2 immunity and is reversible by antibody treatment".SCIENCE 364.6442(2019):751-+. |
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