Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aaw8250 |
Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM | |
Zhao, Yan1; Chen, Shanshuang1; Swensen, Adam C.2; Qian, Wei-Jun2; Gouaux, Eric1,3 | |
2019-04-26 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 364期号:6438页码:355-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Glutamate-gated AMPA receptors mediate the fast component of excitatory signal transduction at chemical synapses throughout all regions of the mammalian brain. AMPA receptors are tetrameric assemblies composed of four subunits, GluA1-GluA4. Despite decades of study, the subunit composition, subunit arrangement, and molecular structure of native AMPA receptors remain unknown. Here we elucidate the structures of 10 distinct native AMPA receptor complexes by single-particle cryo-electron microscopy (cryo-EM). We find that receptor subunits are arranged nonstochastically, with the GluA2 subunit preferentially occupying the B and D positions of the tetramer and with triheteromeric assemblies comprising a major population of native AMPA receptors. Cryo-EM maps define the structure for S2-M4 linkers between the ligand-binding and transmembrane domains, suggesting how neurotransmitter binding is coupled to ion channel gating. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000465643400040 |
WOS关键词 | SYNAPTIC AMPA ; GLUTAMATE RECEPTORS ; TERMINAL DOMAIN ; PHOSPHORYLATION ; DESENSITIZATION ; PLASTICITY ; PROTEINS ; DETERMINANT ; VALIDATION ; MODULATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/201291 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Oregon Hlth & Sci Univ, Vollum Inst, 3181 SW Sam Jackson Pk Rd, Portland, OR 97239 USA; 2.Pacific Northwest Natl Lab, Integrat Omics, Div Biol Sci, Richland, WA 99352 USA; 3.Oregon Hlth & Sci Univ, Howard Hughes Med Inst, 3181 SW Sam Jackson Pk Rd, Portland, OR 97239 USA |
推荐引用方式 GB/T 7714 | Zhao, Yan,Chen, Shanshuang,Swensen, Adam C.,et al. Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM[J]. SCIENCE,2019,364(6438):355-+. |
APA | Zhao, Yan,Chen, Shanshuang,Swensen, Adam C.,Qian, Wei-Jun,&Gouaux, Eric.(2019).Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM.SCIENCE,364(6438),355-+. |
MLA | Zhao, Yan,et al."Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM".SCIENCE 364.6438(2019):355-+. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论