Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aat6457 |
Neutral mass spectrometry of virus capsids above 100 megadaltons with nanomechanical resonators | |
Dominguez-Medina, Sergio1,2,3; Fostner, Shawn4,6; Defoort, Martial4; Sansa, Marc4; Stark, Ann-Kathrin1,2,3; Halim, Mohammad Abdul1,2,3; Vernhes, Emeline5,7; Gely, Marc4; Jourdan, Guillaume4; Alava, Thomas4; Boulanger, Pascale5; Masselon, Christophe1,2,3; Hentz, Sebastien4 | |
2018-11-23 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 362期号:6417页码:918-+ |
文章类型 | Article |
语种 | 英语 |
国家 | France; Canada |
英文摘要 | Measurement of the mass of particles in the mega- to gigadalton range is challenging with conventional mass spectrometry. Although this mass range appears optimal for nanomechanical resonators, nanomechanical mass spectrometers often suffer from prohibitive sample loss, extended analysis time, or inadequate resolution. We report on a system architecture combining nebulization of the analytes from solution, their efficient transfer and focusing without relying on electromagnetic fields, and the mass measurements of individual particles using nanomechanical resonator arrays. This system determined the mass distribution of similar to 30-megadalton polystyrene nanoparticles with high detection efficiency and effectively performed molecular mass measurements of empty or DNA-filled bacteriophage T5 capsids with masses up to 105 megadaltons using less than 1 picomole of sample and with an instrument resolution above 100. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000451124500035 |
WOS关键词 | BACTERIOPHAGE-T5 ; RESOLUTION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/200138 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Grenoble Alpes, F-38000 Grenoble, France; 2.CEA, BIG, Biol Grande Echelle, F-38054 Grenoble, France; 3.Inserm, Unite 1038, F-38054 Grenoble, France; 4.Univ Grenoble Alpes, CEA, LETI, F-38000 Grenoble, France; 5.Univ Paris Saclay, Univ Paris Sud, Inst Integrat Biol Cell I2BC, CEA,CNRS, F-91198 Gif Sur Yvette, France; 6.1891 Robertson Rd,Suite 500, Ottawa, ON K2H 5B7, Canada; 7.Univ Paul Sabatier, Univ Toulouse, CNRS, LMGM,CBI, F-31062 Toulouse, France |
推荐引用方式 GB/T 7714 | Dominguez-Medina, Sergio,Fostner, Shawn,Defoort, Martial,et al. Neutral mass spectrometry of virus capsids above 100 megadaltons with nanomechanical resonators[J]. SCIENCE,2018,362(6417):918-+. |
APA | Dominguez-Medina, Sergio.,Fostner, Shawn.,Defoort, Martial.,Sansa, Marc.,Stark, Ann-Kathrin.,...&Hentz, Sebastien.(2018).Neutral mass spectrometry of virus capsids above 100 megadaltons with nanomechanical resonators.SCIENCE,362(6417),918-+. |
MLA | Dominguez-Medina, Sergio,et al."Neutral mass spectrometry of virus capsids above 100 megadaltons with nanomechanical resonators".SCIENCE 362.6417(2018):918-+. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论