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DOI | 10.1126/science.aav4294 |
Programmed DNA destruction by miniature CRISPR-Cas14 enzymes | |
Harrington, Lucas B.1,9; Burstein, David2,10; Chen, Janice S.1,9; Paez-Espino, David3; Ma, Enbo1; Witte, Isaac P.1; Cofsky, Joshua C.1; Kyrpides, Nikos C.3; Banfield, Jillian F.2,4,5; Doudna, Jennifer A.1,5,6,7,8 | |
2018-11-16 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 362期号:6416页码:839-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Israel |
英文摘要 | CRISPR-Cas systems provide microbes with adaptive immunity to infectious nucleic acids and are widely employed as genome editing tools. These tools use RNA-guided Cas proteins whose large size (950 to 1400 amino acids) has been considered essential to their specific DNA- or RNA-targeting activities. Here we present a set of CRISPR-Cas systems from uncultivated archaea that contain Cas14, a family of exceptionally compact RNA-guided nucleases (400 to 700 amino acids). Despite their small size, Cas14 proteins are capable of targeted single-stranded DNA (ssDNA) cleavage without restrictive sequence requirements. Moreover, target recognition by Cas14 triggers nonspecific cutting of ssDNA molecules, an activity that enables high-fidelity single-nucleotide polymorphism genotyping (Cas14-DETECTR). Metagenomic data show that multiple CRISPR-Cas14 systems evolved independently and suggest a potential evolutionary origin of single-effector CRISPR-based adaptive immunity. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000450488500055 |
WOS关键词 | RNA ; TOOL ; CLASSIFICATION ; DIVERSITY ; EVOLUTION ; CLEAVAGE ; VERSION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/200069 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USA; 2.Univ Calif Berkeley, Dept Earth & Planetary Sci, Berkeley, CA 94720 USA; 3.Joint Genome Inst, Dept Energy, Walnut Creek, CA 94598 USA; 4.Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA; 5.Univ Calif Berkeley, Innovat Genom Inst, Berkeley, CA 94720 USA; 6.Lawrence Berkeley Natl Lab, MBIB Div, Berkeley, CA 94720 USA; 7.Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA; 8.Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA; 9.Mammoth Biosci, San Francisco, CA 94107 USA; 10.Tel Aviv Univ, Sch Mol Cell Biol & Biotechnol, IL-69978 Tel Aviv, Israel |
推荐引用方式 GB/T 7714 | Harrington, Lucas B.,Burstein, David,Chen, Janice S.,et al. Programmed DNA destruction by miniature CRISPR-Cas14 enzymes[J]. SCIENCE,2018,362(6416):839-+. |
APA | Harrington, Lucas B..,Burstein, David.,Chen, Janice S..,Paez-Espino, David.,Ma, Enbo.,...&Doudna, Jennifer A..(2018).Programmed DNA destruction by miniature CRISPR-Cas14 enzymes.SCIENCE,362(6416),839-+. |
MLA | Harrington, Lucas B.,et al."Programmed DNA destruction by miniature CRISPR-Cas14 enzymes".SCIENCE 362.6416(2018):839-+. |
条目包含的文件 | 条目无相关文件。 |
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