Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aar4362 |
Single-cell mapping of gene expression landscapes and lineage in the zebrafish embryo | |
Wagner, Daniel E.; Weinreb, Caleb; Collins, Zach M.; Briggs, James A.; Megason, Sean G.; Klein, Allon M. | |
2018-06-01 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 360期号:6392页码:981-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | High-throughput mapping of cellular differentiation hierarchies from single-cell data promises to empower systematic interrogations of vertebrate development and disease. Here we applied single-cell RNA sequencing to >92,000 cells from zebrafish embryos during the first day of development. Using a graph-based approach, we mapped a cell-state landscape that describes axis patterning, germ layer formation, and organogenesis. We tested how clonally related cells traverse this landscape by developing a transposon-based barcoding approach (TracerSeq) for reconstructing single-cell lineage histories. Clonally related cells were often restricted by the state landscape, including a case in which two independent lineages converge on similar fates. Cell fates remained restricted to this landscape in embryos lacking the chordin gene. We provide web-based resources for further analysis of the single-cell data. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000433574200034 |
WOS关键词 | NEURAL CREST ; FATE MAP ; CHORDIN ; TAILBUD ; SPECIFICATION ; GASTRULATION ; PROGRESSION ; INHIBITION ; PLASTICITY ; ORIGIN |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/198797 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA |
推荐引用方式 GB/T 7714 | Wagner, Daniel E.,Weinreb, Caleb,Collins, Zach M.,et al. Single-cell mapping of gene expression landscapes and lineage in the zebrafish embryo[J]. SCIENCE,2018,360(6392):981-+. |
APA | Wagner, Daniel E.,Weinreb, Caleb,Collins, Zach M.,Briggs, James A.,Megason, Sean G.,&Klein, Allon M..(2018).Single-cell mapping of gene expression landscapes and lineage in the zebrafish embryo.SCIENCE,360(6392),981-+. |
MLA | Wagner, Daniel E.,et al."Single-cell mapping of gene expression landscapes and lineage in the zebrafish embryo".SCIENCE 360.6392(2018):981-+. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论