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DOI | 10.1126/science.aap7847 |
Uncovering the essential genes of the human malaria parasite Plasmodium falciparum by saturation mutagenesis | |
Zhang, Min1; Wang, Chengqi1; Otto, Thomas D.2,3; Oberstaller, Jenna1; Liao, Xiangyun1; Adapa, Swamy R.1; Udenze, Kenneth1; Bronner, Iraad F.2; Casandra, Deborah1; Mayho, Matthew2; Brown, Jacqueline2; Li, Suzanne1; Swanson, Justin1; Rayner, Julian C.2; Jiang, Rays H. Y.1; Adams, John H.1 | |
2018-05-04 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 360期号:6388页码:506-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA; England; Scotland |
英文摘要 | Severe malaria is caused by the apicomplexan parasite Plasmodium falciparum. Despite decades of research, the distinct biology of these parasites has made it challenging to establish high-throughput genetic approaches to identify and prioritize therapeutic targets. Using transposon mutagenesis of P. falciparum in an approach that exploited its AT-rich genome, we generated more than 38,000 mutants, saturating the genome and defining mutability and fitness costs for over 87% of genes. Of 5399 genes, our study defined 2680 genes as essential for optimal growth of asexual blood stages in vitro. These essential genes are associated with drug resistance, represent leading vaccine candidates, and include approximately 1000 Plasmodium-conserved genes of unknown function. We validated this approach by testing proteasome pathways for individualmutants associated with artemisinin sensitivity. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000431351500038 |
WOS关键词 | NUCLEAR POLYHEDROSIS VIRUSES ; ARTEMISININ RESISTANCE ; TRANSPOSON MUTAGENESIS ; GENOME ; BACULOVIRUSES ; INSERTIONS ; PIGGYBAC ; MUTANTS ; PROTEIN ; SPREAD |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/198577 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ S Florida, Dept Global Hlth, Ctr Global Hlth & Infect Dis, 3720 Spectrum Blvd,Suite 404, Tampa, FL 33612 USA; 2.Wellcome Trust Sanger Inst, Malaria Programme, Genome Campus, Hinxton CB10 1SA, Cambs, England; 3.Univ Glasgow, Coll Med Vet & Life Sci, Ctr Immunobiol, Inst Infect Immun & Inflammat, Glasgow, Lanark, Scotland |
推荐引用方式 GB/T 7714 | Zhang, Min,Wang, Chengqi,Otto, Thomas D.,et al. Uncovering the essential genes of the human malaria parasite Plasmodium falciparum by saturation mutagenesis[J]. SCIENCE,2018,360(6388):506-+. |
APA | Zhang, Min.,Wang, Chengqi.,Otto, Thomas D..,Oberstaller, Jenna.,Liao, Xiangyun.,...&Adams, John H..(2018).Uncovering the essential genes of the human malaria parasite Plasmodium falciparum by saturation mutagenesis.SCIENCE,360(6388),506-+. |
MLA | Zhang, Min,et al."Uncovering the essential genes of the human malaria parasite Plasmodium falciparum by saturation mutagenesis".SCIENCE 360.6388(2018):506-+. |
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