Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aam8999 |
Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding | |
Rosenberg, Alexander B.1; Roco, Charles M.2; Muscat, Richard A.1; Kuchina, Anna1; Sample, Paul1; Yao, Zizhen3; Graybuck, Lucas T.3; Peeler, David J.2; Mukherjee, Sumit1; Chen, Wei4; Pun, Suzie H.2; Sellers, Drew L.2,5; Tasic, Bosiljka3; Seelig, Georg1,4,6 | |
2018-04-13 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 360期号:6385页码:176-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | To facilitate scalable profiling of single cells, we developed split-pool ligation-based transcriptome sequencing (SPLiT-seq), a single-cell RNA-seq (scRNA-seq) method that labels the cellular origin of RNA through combinatorial barcoding. SPLiT-seq is compatible with fixed cells or nuclei, allows efficient sample multiplexing, and requires no customized equipment. We used SPLiT-seq to analyze 156,049 single-nucleus transcriptomes from postnatal day 2 and 11 mouse brains and spinal cords. More than 100 cell types were identified, with gene expression patterns corresponding to cellular function, regional specificity, and stage of differentiation. Pseudotime analysis revealed transcriptional programs driving four developmental lineages, providing a snapshot of early postnatal development in the murine central nervous system. SPLiT-seq provides a path toward comprehensive single-cell transcriptomic analysis of other similarly complex multicellular systems. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000429805400040 |
WOS关键词 | RNA-SEQ ; CEREBELLAR CORTEX ; MOTOR-NEURONS ; EXPRESSION ; TRANSCRIPTOMICS ; HIPPOCAMPUS ; DIVERSITY ; INTERNEURONS ; PRECURSORS ; SUBTYPES |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/198417 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Washington, Dept Elect Engn, Seattle, WA 98195 USA; 2.Univ Washington, Dept Bioengn, Seattle, WA 98195 USA; 3.Allen Inst Brain Sci, Seattle, WA USA; 4.Univ Washington, Mol Engn & Sci Inst, Seattle, WA 98195 USA; 5.Inst Stem Cell & Regenerat Med, Seattle, WA USA; 6.Univ Washington, Paul G Allen Sch Comp Sci & Engn, Seattle, WA 98195 USA |
推荐引用方式 GB/T 7714 | Rosenberg, Alexander B.,Roco, Charles M.,Muscat, Richard A.,et al. Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding[J]. SCIENCE,2018,360(6385):176-+. |
APA | Rosenberg, Alexander B..,Roco, Charles M..,Muscat, Richard A..,Kuchina, Anna.,Sample, Paul.,...&Seelig, Georg.(2018).Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding.SCIENCE,360(6385),176-+. |
MLA | Rosenberg, Alexander B.,et al."Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding".SCIENCE 360.6385(2018):176-+. |
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