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| DOI | 10.1126/science.aan8414 |
| 3.9 angstrom structure of the yeast Mec1-Ddc2 complex, a homolog of human ATR-ATRIP | |
| Wang, Xuejuan1,2,3; Ran, Tingting4; Zhang, Xuan1,2; Xin, Jiyu1,2; Zhang, Zhihui1,2; Wu, Tengwei1,2; Wang, Weiwu4; Cai, Gang1,2,3 | |
| 2017-12-01 | |
| 发表期刊 | SCIENCE
 |
| ISSN | 0036-8075 |
| EISSN | 1095-9203 |
| 出版年 | 2017 |
| 卷号 | 358期号:6367页码:1206-1209 |
| 文章类型 | Article |
| 语种 | 英语 |
| 国家 | Peoples R China |
| 英文摘要 | The ataxia telangiectasia-mutated and Rad3-related (ATR) kinase is a master regulator of DNA damage response and replication stress in humans, but the mechanism of its activation remains unclear. ATR acts together with its partner ATRIP. Using cryo-electron microscopy, we determined the structure of intact Mec1-Ddc2 (the yeast homolog of ATR-ATRIP), which is poised for catalysis, at a resolution of 3.9 angstroms. Mec1-Ddc2 forms a dimer of heterodimers through the PRD and FATdomains of Mec1 and the coiled-coil domain of Ddc2. The PRD and Bridge domains in Mec1 constitute critical regulatory sites. The activation loop of Mec1 is inhibited by the PRD, revealing an allosteric mechanism of kinase activation. Our study clarifies the architecture of ATR-ATRIP and provides a structural framework for the understanding of ATR regulation. |
| 领域 | 地球科学 ; 气候变化 ; 资源环境 |
| 收录类别 | SCI-E |
| WOS记录号 | WOS:000416584000046 |
| WOS关键词 | STALLED REPLICATION FORKS ; ATAXIA-TELANGIECTASIA ; CHECKPOINT ACTIVATION ; DNA-DAMAGE ; PROTEIN ; KINASE ; ARCHITECTURE ; MEC1(ATR) |
| WOS类目 | Multidisciplinary Sciences |
| WOS研究方向 | Science & Technology - Other Topics |
| URL | 查看原文 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/197446 |
| 专题 | 地球科学 资源环境科学 气候变化 |
| 作者单位 | 1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China; 2.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China; 3.Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Hefei 230026, Anhui, Peoples R China; 4.Nanjing Agr Univ, Key Lab Agr & Environm Microbiol, Minist Agr, Coll Life Sci, Nanjing 210095, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xuejuan,Ran, Tingting,Zhang, Xuan,et al. 3.9 angstrom structure of the yeast Mec1-Ddc2 complex, a homolog of human ATR-ATRIP[J]. SCIENCE,2017,358(6367):1206-1209. |
| APA | Wang, Xuejuan.,Ran, Tingting.,Zhang, Xuan.,Xin, Jiyu.,Zhang, Zhihui.,...&Cai, Gang.(2017).3.9 angstrom structure of the yeast Mec1-Ddc2 complex, a homolog of human ATR-ATRIP.SCIENCE,358(6367),1206-1209. |
| MLA | Wang, Xuejuan,et al."3.9 angstrom structure of the yeast Mec1-Ddc2 complex, a homolog of human ATR-ATRIP".SCIENCE 358.6367(2017):1206-1209. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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