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DOI | 10.1126/science.aak9787 |
Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion (LIANTI) | |
Chen, Chongyi1; Xing, Dong1; Tan, Longzhi1; Li, Heng2; Zhou, Guangyu1; Huang, Lei1,3,4; Xie, X. Sunney1,3,4 | |
2017-04-14 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 356期号:6334页码:189-194 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Peoples R China |
英文摘要 | Single-cell genomics is important for biology and medicine. However, current whole-genome amplification (WGA) methods are limited by low accuracy of copy-number variation (CNV) detection and low amplification fidelity. Here we report an improved single-cell WGA method, Linear Amplification via Transposon Insertion (LIANTI), which outperforms existing methods, enabling micro-CNV detection with kilobase resolution. This allowed direct observation of stochastic firing of DNA replication origins, which differs from cell to cell. We also show that the predominant cytosine-to-thymine mutations observed in single-cell genomics often arise from the artifact of cytosine deamination upon cell lysis. However, identifying single-nucleotide variations (SNVs) can be accomplished by sequencing kindred cells. We determined the spectrum of SNVs in a single human cell after ultraviolet radiation, revealing their nonrandom genome-wide distribution. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000399013800037 |
WOS关键词 | DNA-DAMAGE ; CHROMATIN ORGANIZATION ; EXCISION-REPAIR ; MUTATION-RATES ; TRANSCRIPTION ; NUCLEOTIDE ; STRAND ; RECOMBINATION ; DEAMINATION ; EVOLUTION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/195817 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA; 2.Massachusetts Inst Technol MIT & Harvard, Broad Inst, Cambridge, MA 02142 USA; 3.Peking Univ, Sch Life Sci, Biodynam Opt Imaging Ctr BIOPIC, Beijing 100871, Peoples R China; 4.Peking Univ, Beijing Adv Innovat Ctr Genom ICG, Beijing 100871, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Chongyi,Xing, Dong,Tan, Longzhi,et al. Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion (LIANTI)[J]. SCIENCE,2017,356(6334):189-194. |
APA | Chen, Chongyi.,Xing, Dong.,Tan, Longzhi.,Li, Heng.,Zhou, Guangyu.,...&Xie, X. Sunney.(2017).Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion (LIANTI).SCIENCE,356(6334),189-194. |
MLA | Chen, Chongyi,et al."Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion (LIANTI)".SCIENCE 356.6334(2017):189-194. |
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