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DOI | 10.1126/science.aal3059 |
Architecture of a transcribing-translating expressome | |
Kohler, R.1; Mooney, R. A.2; Mills, D. J.3; Landick, R.2; Cramer, P.1 | |
2017-04-14 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 356期号:6334页码:194-197 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany; USA |
英文摘要 | DNA transcription is functionally coupled to messenger RNA (mRNA) translation in bacteria, but how this is achieved remains unclear. Here we show that RNA polymerase (RNAP) and the ribosome of Escherichia coli can form a defined transcribing and translating "expressome" complex. The cryo-electron microscopic structure of the expressome reveals continuous protection of similar to 30 nucleotides of mRNA extending from the RNAP active center to the ribosome decoding center. The RNAP-ribosome interface includes the RNAP subunit a carboxyl-terminal domain, which is required for RNAP-ribosome interaction in vitro and for pronounced cell growth defects upon translation inhibition in vivo, consistent with its function in transcription-translation coupling. The expressome structure can only form during transcription elongation and explains how translation can prevent transcriptional pausing, backtracking, and termination. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000399013800038 |
WOS关键词 | BACTERIAL RNA-POLYMERASE ; STRUCTURAL BASIS ; ALPHA-SUBUNIT ; TRANSCRIPTION ELONGATION ; ELECTRON CRYOMICROSCOPY ; ANGSTROM RESOLUTION ; CRYSTAL-STRUCTURE ; COMPLEX ; RIBOSOME ; ACTIVATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/195812 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Max Planck Inst Biophys Chem, Dept Mol Biol, Fassberg 11, D-37077 Gottingen, Germany; 2.Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA; 3.Max Planck Inst Biophys, Dept Biol Struct, Max von Laue Str 3, D-60438 Frankfurt, Germany |
推荐引用方式 GB/T 7714 | Kohler, R.,Mooney, R. A.,Mills, D. J.,et al. Architecture of a transcribing-translating expressome[J]. SCIENCE,2017,356(6334):194-197. |
APA | Kohler, R.,Mooney, R. A.,Mills, D. J.,Landick, R.,&Cramer, P..(2017).Architecture of a transcribing-translating expressome.SCIENCE,356(6334),194-197. |
MLA | Kohler, R.,et al."Architecture of a transcribing-translating expressome".SCIENCE 356.6334(2017):194-197. |
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