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DOI | 10.1126/science.aaf4791 |
Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome | |
Shen, Yue1,2,10,11; Wang, Yun1,11; Chen, Tai1,11; Gao, Feng1; Gong, Jianhui1; Abramczyk, Dariusz2; Walker, Roy2; Zhao, Hongcui1; Chen, Shihong1; Liu, Wei2; Luo, Yisha2; Mueller, Carolin A.3; Paul-Dubois-Taine, Adrien2; Alver, Bonnie2; Stracquadanio, Giovanni4,5,12; Mitchell, Leslie A.6; Luo, Zhouqing7; Fan, Yanqun1; Zhou, Baojin1; Wen, Bo1; Tan, Fengji1; Wang, Yujia1; Zi, Jin1; Xie, Zexiong8; Li, Bingzhi8; Yang, Kun4; Richardson, Sarah M.4,5; Jiang, Hui1; French, Christopher E.2; Nieduszynski, Conrad A.3; Koszul, Romain9; Marston, Adele L.2; Yuan, Yingjin8; Wang, Jian1; Bader, Joel S.5,6; Dai, Junbiao7; Boeke, Jef D.6; Xu, Xun1,11; Cai, Yizhi2; Yang, Huanming1,10 | |
2017-03-10 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 355期号:6329 |
文章类型 | Article |
语种 | 英语 |
国家 | Peoples R China; Scotland; England; USA; France |
英文摘要 | Here, we report the successful design, construction, and characterization of a 770-kilobase synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels-including phenomics, transcriptomics, proteomics, chromosome segregation, and replication analysis-to provide a thorough and comprehensive analysis of a synthetic chromosome. Our Trans-Omics analyses reveal a modest but potentially relevant pervasive up-regulation of translational machinery observed in synII, mainly caused by the deletion of 13 transfer RNAs. By both complementation assays and SCRaMbLE (synthetic chromosome rearrangement and modification by loxP-mediated evolution), we targeted and debugged the origin of a growth defect at 37 degrees C in glycerol medium, which is related to misregulation of the high-osmolarity glycerol response. Despite the subtle differences, the synII strain shows highly consistent biological processes comparable to the native strain. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000396348900034 |
WOS关键词 | MYCOPLASMA-GENITALIUM GENOME ; TRANSFER-RNA DECAY ; SACCHAROMYCES-CEREVISIAE ; SEQUENCING DATA ; GENES ; REPLICATION ; DUPLICATION ; EXPLORATION ; CLONING ; PATHWAY |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/195582 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.BGI Shenzhen, Shenzhen 518083, Peoples R China; 2.Univ Edinburgh, Sch Biol Sci, Edinburgh EH9 3BF, Midlothian, Scotland; 3.Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England; 4.Johns Hopkins Univ, Sch Med, High Throughput Biol Ctr, Baltimore, MD 21205 USA; 5.Johns Hopkins Univ, Sch Engn, Dept Biomed Engn, Baltimore, MD 21218 USA; 6.NYU, Langone Med Ctr, Inst Syst Genet, ACLSW Room 503,430 East 29th St, New York, NY 10016 USA; 7.Tsinghua Univ, Key Lab Ind Biocatalysis, Minist Educ, Ctr Synthet & Syst Biol,Sch Life Sci, Beijing 100084, Peoples R China; 8.Tianjin Univ, Key Lab Syst Bioengn, Minist Educ,Sch Chem Engn & Technol, SynBio Res Platform,Collaborat Innovat Ctr Chem S, Tianjin 300072, Peoples R China; 9.Inst Pasteur, CNRS, UMR3525, Dept Genomes & Genet, 25-28 Rue Docteur Roux, F-75724 Paris 15, France; 10.James D Watson Inst Genome Sci, Hangzhou 310058, Zhejiang, Peoples R China; 11.BGI Qingdao, Qingdao 266555, Peoples R China; 12.Univ Essex, Sch Comp Sci & Elect Engn, Wivenhoe Pk, Colchester CO4 3SQ, Essex, England |
推荐引用方式 GB/T 7714 | Shen, Yue,Wang, Yun,Chen, Tai,et al. Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome[J]. SCIENCE,2017,355(6329). |
APA | Shen, Yue.,Wang, Yun.,Chen, Tai.,Gao, Feng.,Gong, Jianhui.,...&Yang, Huanming.(2017).Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome.SCIENCE,355(6329). |
MLA | Shen, Yue,et al."Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome".SCIENCE 355.6329(2017). |
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