Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond

doi:10.1126/science.aaf4831

GSTDTAP > 地球科学

DOI	10.1126/science.aaf4831
	Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond
	Mitchell, Leslie A.1,2; Wang, Ann 3,4; Stracquadanio, Giovanni 3,5,6,7; Kuang, Zheng 1,2; Wang, Xuya 1,2; Yang, Kun 3,5,6; Richardson, Sarah 3,5,6; Martin, J. Andrew 1,2; Zhao, Yu 1,2; Walker, Roy 8; Luo, Yisha 8; Dai, Hongjiu 9; Dong, Kang 9; Tang, Zuojian 1,2; Yang, Yanling 10; Cai, Yizhi 8; Heguy, Adriana 11; Ueberheide, Beatrix 1,10; Fenyo, David 1,2; Dai, Junbiao 4; Bader, Joel S.3,4; Boeke, Jef D.1,2
	2017-03-10
发表期刊	SCIENCE
ISSN	0036-8075
EISSN	1095-9203
出版年	2017
卷号	355 期号:6329
文章类型	Article
语种	英语
国家	USA; Peoples R China; England; Scotland
英文摘要	We describe design, rapid assembly, and characterization of synthetic yeast Sc2.0 chromosome VI (synVI). A mitochondrial defect in the synVI strain mapped to synonymous coding changes within PRE4 (YFR050C), encoding an essential proteasome subunit; Sc2.0 coding changes reduced Pre4 protein accumulation by half. Completing Sc2.0 specifies consolidation of 16 synthetic chromosomes into a single strain. We investigated phenotypic, transcriptional, and proteomewide consequences of Sc2.0 chromosome consolidation in poly-synthetic strains. Another "bug" was discovered through proteomic analysis, associated with alteration of the HIS2 transcription start due to transfer RNA deletion and loxPsym site insertion. Despite extensive genetic alterations across 6% of the genome, no major global changes were detected in the poly-synthetic strain " omics" analyses. This work sets the stage for completion of a designer, synthetic eukaryotic genome.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000396348900032
WOS关键词	SACCHAROMYCES-CEREVISIAE ; YEAST ; DNA ; PROTEASOME ; PCR ; DELETION ; SUBUNIT ; STRAINS ; LONG ; ACTS
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
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引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/195581
专题	地球科学资源环境科学气候变化
作者单位	1.NYU, Langone Sch Med, Dept Biochem & Mol Pharmacol, 550 1St Ave, New York, NY 10016 USA; 2.NYU, Langone Sch Med, Inst Syst Genet, 550 1St Ave, New York, NY 10016 USA; 3.Johns Hopkins Univ, Sch Med, High Throughput Biol Ctr, Baltimore, MD 21205 USA; 4.Tsinghua Univ, Key Lab Ind Biocatalysis, Minist Educ, Key Lab Bioinformat,Ctr Synthet & Syst Biol,Sch L, Beijing 100084, Peoples R China; 5.Johns Hopkins Univ, Whiting Sch Engn, Dept Biomed Engn, Baltimore, MD 21218 USA; 6.Johns Hopkins Univ, Whiting Sch Engn, Inst Med Genet, Baltimore, MD 21218 USA; 7.Univ Essex, Sch Comp Sci & Elect Engn, Wivenhoe Pk, Colchester CO4 3SQ, Essex, England; 8.Univ Edinburgh, Sch Biol Sci, Ctr Synthet & Syst Biol, Edinburgh EH9 3JL, Midlothian, Scotland; 9.GenScript, Piscataway, NJ 08854 USA; 10.NYU, Langone Sch Med, Prote Resource Ctr, Off Collaborat Sci, 550 1St Ave, New York, NY 10016 USA; 11.NYU, Langone Sch Med, Genome Technol Ctr, 550 1St Ave, New York, NY 10016 USA
推荐引用方式 GB/T 7714	Mitchell, Leslie A.,Wang, Ann,Stracquadanio, Giovanni,et al. Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond[J]. SCIENCE,2017,355(6329).
APA	Mitchell, Leslie A..,Wang, Ann.,Stracquadanio, Giovanni.,Kuang, Zheng.,Wang, Xuya.,...&Boeke, Jef D..(2017).Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond.SCIENCE,355(6329).
MLA	Mitchell, Leslie A.,et al."Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond".SCIENCE 355.6329(2017).