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DOI | 10.1126/science.aah4077 |
Experimental measurement of binding energy, selectivity, and allostery using fluctuation theorems | |
Camunas-Soler, Joan1,2,3,4; Alemany, Anna1,2,5,6; Ritort, Felix1,2 | |
2017-01-27 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 355期号:6323页码:412-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Spain; USA; Netherlands |
英文摘要 | Thermodynamic bulk measurements of binding reactions rely on the validity of the law of mass action and the assumption of a dilute solution. Yet, important biological systems such as allosteric ligand-receptor binding, macromolecular crowding, or misfolded molecules may not follow these assumptions and may require a particular reaction model. Here we introduce a fluctuation theorem for ligand binding and an experimental approach using single-molecule force spectroscopy to determine binding energies, selectivity, and allostery of nucleic acids and peptides in a model-independent fashion. A similar approach could be used for proteins. This work extends the use of fluctuation theorems beyond unimolecular folding reactions, bridging the thermodynamics of small systems and the basic laws of chemical equilibrium. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000393172800043 |
WOS关键词 | PROTEIN-DNA INTERACTIONS ; MECHANICAL STABILITY ; ECHINOMYCIN BINDING ; MOLECULE ; COOPERATIVITY ; ENDONUCLEASE ; EQUILIBRIUM ; RECOGNITION ; SPECIFICITY ; ENERGETICS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/195312 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Barcelona, Fac Fis, Dept Fis Mat Condensada, Small Biosyst Lab, Barcelona, Spain; 2.Inst Salud Carlos III, Ctr Invest Biomed Red CIBER Bioingn Biomat & Nano, Madrid, Spain; 3.Stanford Univ, Dept Bioengn, Stanford, CA 94304 USA; 4.Stanford Univ, Dept Appl Phys, Stanford, CA 94304 USA; 5.Royal Netherlands Acad Arts & Sci KNAW, Hubrecht Inst, NL-3584 CT Utrecht, Netherlands; 6.Univ Med Ctr Utrecht, NL-3584 CT Utrecht, Netherlands |
推荐引用方式 GB/T 7714 | Camunas-Soler, Joan,Alemany, Anna,Ritort, Felix. Experimental measurement of binding energy, selectivity, and allostery using fluctuation theorems[J]. SCIENCE,2017,355(6323):412-+. |
APA | Camunas-Soler, Joan,Alemany, Anna,&Ritort, Felix.(2017).Experimental measurement of binding energy, selectivity, and allostery using fluctuation theorems.SCIENCE,355(6323),412-+. |
MLA | Camunas-Soler, Joan,et al."Experimental measurement of binding energy, selectivity, and allostery using fluctuation theorems".SCIENCE 355.6323(2017):412-+. |
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